Skip to main content
ARS Home » Northeast Area » Orient Point, New York » Plum Island Animal Disease Center » Foreign Animal Disease Research » People & Locations » Manuel Borca

Manuel Borca (PhD)
Foreign Animal Disease Research
Research Microbiologist

Phone: (631) 323-3019
Fax: (631) 323-3006

40550 ROUTE 25
USDA ORIENT POINT WAREHOUSE
ORIENT POINT, NY 11957
Projects
Countermeasures to Control and Eradicate Foreign Animal Diseases of Swine
In-House Appropriated (D)
  Accession Number: 431897
Intervention Strategies to Support the Global Control and Eradication of Foot-and-Mouth Disease Virus (FMDV)
In-House Appropriated (D)
  Accession Number: 431980
Alternative Approaches for the Development of an Experimental African Swine Fever Virus (ASFV) Vaccine
Interagency Reimbursable Agreement (I)
  Accession Number: 428061
Development of a Live Attenuated African Swine Fever Virus Vaccine With DIVA Capability
Interagency Reimbursable Agreement (I)
  Accession Number: 434896
A DIVA Compatible Vaccine Against Classical Swine Fever (CSF) Made in the USA
Interagency Reimbursable Agreement (I)
  Accession Number: 434923
Systems Biology Approach to Understand Mechanisms of Immune Protection Against African Swine Fever Virus Infection
Interagency Reimbursable Agreement (I)
  Accession Number: 436777
Use of Monoclonal Antibodies to Study Interaction Between Foot-and-Mouth Disease Virus and Host Proteins During Infection
Non-Funded Cooperative Agreement (N)
  Accession Number: 432398
Development of novel and/or improved vaccines strains of Classical Swine Fever Virus (CSFV) and African Swine Fever Virus (ASFV)
Non-Funded Cooperative Agreement (N)
  Accession Number: 432586
Foreign Animal Disease Research and Development Activities to Address High Priority Transboundary Animal Diseases
Non-Funded Cooperative Agreement (N)
  Accession Number: 432658
Development of a Next-Generation African Swine Fever Vaccine using Bioinformatic Analysis of Circulating Strains in Uganda
Non-Funded Cooperative Agreement (N)
  Accession Number: 434404
Novel Pseudorabies Virus (PRV) Vectored Bivalent Vaccine Against Classical Swine Fever and Porcine Circo Viruses
Reimbursable Cooperative Agreement (R)
  Accession Number: 437677
Characterizing the Structure-Function of Various Families of Viroporins to Support African Swine Fever Virus Vaccine Candidates
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438233
Development of Synthetic African Swine Fever Viruses to Determine the Role of Structural Viral Proteins
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438304
CRISPR KO and CRISPRa Libraries for Creating Stable Cell Lines for Use in African Swine Fever Virus Vaccines
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438358
Identification of Host Proteins That Interact with ASFV Proteins Through Yeast Two Hybrid Screening
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438473
Analysis of the Immune Response Elicited by Classical Swine Fever Virus Recombinant Vaccine Candidate FlagT4G
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438599
Understanding Cross-Reactive Responses Between Heterologous Foot-and-Mouth Disease Virus Strains in the Bovine Model
Non-Assistance Cooperative Agreement (S)
  Accession Number: 434560
Development of Novel Countermeasures Against Swine Diseases
Non-Assistance Cooperative Agreement (S)
  Accession Number: 432876
Role of the Local and Systemic Immunity in the Protection Against Virulent FMDV Challenge by the Oronasal Route in Cattle
Non-Assistance Cooperative Agreement (S)
  Accession Number: 431128
Predictive Structure of African Swine Fever Viral Proteins
Non-Assistance Cooperative Agreement (S)
  Accession Number: 433925
Improved Immunogenic Vectors for Classical Swine Fever and Porcine Reproduction and Respository Syndrom Viruses
Non-Assistance Cooperative Agreement (S)
  Accession Number: 434162
Identification of Critical Host Genes for African Swine Fever Virus Infection Using Genome-Scale CRISPR Knock-Out Cell Libraries
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436791
Actions Supporting the Development of an African Swine Fever Virus Live Attenuated DIVA Vaccine
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436793
Genetic Characterization of African Swine Fever Virus Causing Disease Outbreaks in Asia and Africa
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436802
Development of an African Swine Fever Virus Subunit Vaccine
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436806
Functional Identification of CD8 T-cell Specific Determinants in ASFV Georgia 2007/1 Isolate
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436955
Research Supporting Workforce Development for the National Bio-Agro Defense Facility: Development of CSF Subunit Vaccines
Non-Assistance Cooperative Agreement (S)
  Accession Number: 435093
Analysis of the Interaction Between Virus Proteins and Membrane Structures in Cells Infected with Classical Swine Fever Virus (CSFV)
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436252
Development of Recombinant Porcine Cytomegalovirus (pCMV)- vaccine vector(s) using Korean pCMV field isolates
Trust Fund Cooperative Agreement (T)
  Accession Number: 433645

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E.A., Pruitt, S., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine. Viruses. https://doi.org/10.3390/v12090918.
The C962R ORF of African swine fever strain Georgia is non-essential and not required for virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E.A., Pruitt, S., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. The C962R ORF of African swine fever strain Georgia is non-essential and not required for virulence in swine. Viruses. https://doi.org/10.3390/v12060676.
A Single Amino Acid Substitution In The Matrix Protein (M51R) Of Vesicular Stomatitis New Jersey Virus Impairs Replication In Cultured Porcine Macrophages And Results In Significant Attenuation In Pigs Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Pauszek, S.J., Holinka, L.G., Gladue, D.P., Rekant, S.I., Bishop, E.A., Stenfeldt, C., Verdugo-Rodriguez, A., Borca, M.V., Rodriguez, L.L., Arzt, J. 2020. A Single Amino Acid Substitution In The Matrix Protein (M51R) Of Vesicular Stomatitis New Jersey Virus Impairs Replication In Cultured Porcine Macrophages And Results In Significant Attenuation In Pigs. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2020.01123.
SERTA domain containing protein 1 (SERTAD1) interacts with classical swine fever virus structural glycoprotein E2 which is involved in virus virulence in swine Reprint Icon - (Peer Reviewed Journal)
Vuono, E., Ramirez-Medina, E., Azzinaro, P.A., Berggren, K., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Borca, M.V., Gladue, D.P. 2020. SERTA domain containing protein 1 (SERTAD1) interacts with classical swine fever virus structural glycoprotein E2 which is involved in virus virulence in swine. Journal of Virology. https://doi.org/10.3390/v12040421.
Swine host protein coiled-coil domain containing 115 (CCDC115) interacts with classical swine fever virus structural glycoprotein E2 during virus Reprint Icon - (Peer Reviewed Journal)
Vuonno, E., Ramirez-Media, E., Berggren, K., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. Swine host protein coiled-coil domain containing 115 (CCDC115) interacts with classical swine fever virus structural glycoprotein E2 during virus. Viruses. https://doi.org/10.3390/v12040388.
The MGF360-16R ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host proteins SERTAD3 and SDCBP Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Silva, E., Rai, A., Pruitt, S.E., Vuono, E.A., Velazquez-Salinas, L., Berggren, K.A., Zhu, J.J., Borca, M.V., Gladue, D.P. 2020. The MGF360-16R ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host proteins SERTAD3 and SDCBP. Viruses. https://doi.org/10.1128/JVI.02017-19.
Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine. Reprint Icon - (Abstract Only)
Borca, M.V., Ramirez Media, E., Silva, E., Vuono, E., Rai, A., Pruitt, S., Holinka, L., Velazquezsalinas, L., Gladue, D.P., Risatti, G., Shi, J. 2020. Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine.. Meeting Abstract. https://doi.org/10.1038/s41598-020-57455-3.
Mechanisms of African Swine Fever Virus Pathogenesis and Immune Evasion Inferred from Gene Expression Changes in Infected Swine Macrophages - (Peer Reviewed Journal)
Structural glycoprotein E2 of classical swine fever virus binding to host protein dynactin subunit (DCTN6) is necessary for virus virulence in swine Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Vuono, E.A., Ramirez-Medina, E., Azzinaro, P.A., Berggren, K.A., Singer, M., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Carrillo, C., Gladue, D.P. 2019. Structural glycoprotein E2 of classical swine fever virus binding to host protein dynactin subunit (DCTN6) is necessary for virus virulence in swine. Journal of Virology. https://doi.org/10.1128/JVI.01642-19.
The differential effect of the deletion of African swine fever virus virulence-associated genes in the induction of attenuation of the highly virulent Georgia strain Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E., O'Donnell, V., Holinka, L.G., Silva, E., Rai, A., Pruitt, S., Carrillo, C., Gladue, D.P., Borca, M.V. 2019. The differential effect of the deletion of African swine fever virus virulence-associated genes in the induction of attenuation of the highly virulent Georgia strain. Virology. https://doi.org/10.3390/v11070599.
The role of interleukin 6 during viral infections Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Verdugo-Rodriguez, A., Rodriguez, L.L., Borca, M.V. 2019. The role of interleukin 6 during viral infections. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2019.01057.
Interaction of structural glycoprotein E2 of Classical Swine Fever Virus with protein phosphatase 1 catalytic subunit beta (PP1ß) - (Peer Reviewed Journal)
Vuono, E., Ramirez-Medina, E., Holinka-Patterson, L.G., Baker-Branstetter, R., Borca, M.V., Gladue, D.P. 2019. Interaction of structural glycoprotein E2 of Classical Swine Fever Virus with protein phosphatase 1 catalytic subunit beta (PP1ß). Virology. Viruses 2019, 11(4), 307. https://doi.org/10.3390/v11040307.
Validation of a site-specific recombination cloning technique for the rapid development of an infectious cDNA clone of a virulent field strain of vesicular stomatitis New Jersey virus Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Pauszek, S.J., Barrera, J., Clark, B., Borca, M.V., Verdugo-Rodriguez, A., Arzt, J., Rodriguez, L.L. 2019. Validation of a site-specific recombination cloning technique for the rapid development of an infectious cDNA clone of a virulent field strain of vesicular stomatitis New Jersey virus. Journal of Virological Methods. Volume 265, March 2019, Pages 113-116. https://doi.org/10.1016/j.jviromet.2019.01.003.
Identification of structural glycoprotein E2 domain critical to mediate Classical Swine Fever Virus replication in SK6 cells Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Holinka-Patterson, L.G., Ramirez-Medina, E., Vuono, E., Berggren, K., Gladue, D.P. 2018. Identification of structural glycoprotein E2 domain critical to mediate Classical Swine Fever Virus replication in SK6 cells. Virology. 526:38-44. https://doi.org/10.1016/j.virol.2018.10.004.
Molecular characterization of the viroporin function of foot-and-mouth disease virus non-structureal protein 2B Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., Largo, E., De La Arada, I., Aguilella, V., Alcaraz, A., Arrondo, J., Holinka-Patterson, L.G., Brocchi, E., Ramirez-Medina, E., Vuono, E., Berggren, K., Carrillo, C., Borca, M.V. 2018. Molecular characterization of the viroporin function of foot-and-mouth disease virus non-structureal protein 2B. Journal of Virology. https://doi.org/10.1128/JVI.01360-18.
Classical swine fever virus p7 protein interacts with host protein CAMLG and regulates calcium permeability at the endoplasmic reticulum Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., Largo, E., Holinka-Patterson, L.G., Ramirez-Medina, E., Vuono, E.A., Berggren, K., Risatti, G.R., Nieva, J.L., Borca, M.V. 2018. Classical swine fever virus p7 protein interacts with host protein CAMLG and regulates calcium permeability at the endoplasmic reticulum. Virus Research. 10(9):E460. https://doi.org/10.3390/v10090460.
Characterization of naturally occurring, acute and persistent subclinical foot-and-mouth disease virus infection in vaccinated Asian buffalo in Pakistan - (Peer Reviewed Journal)
Increased virulence of an epidemic strain of vesicular stomatitis virus is associated with interference of the innate response in pigs Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Pauszek, S.J., Stenfeldt, C., O'Hearn, E.S., Pacheco, J.M., Borca, M.V., Verdugo-Rodriguez, A., Arzt, J., Rodriguez, L.L. 2018. Increased virulence of an epidemic strain of vesicular stomatitis virus is associated with interference of the innate response in pigs. Frontiers in Microbiology. 9:1891. https://doi.org/10.3389/fmicb.2018.01891.
CRISPR/Cas gene editing of a large DNA virus: African Swine Fever Virus Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Berggren, K., Ramirez, E., Vuono, E., Gladue, D.P. 2018. CRISPR/Cas gene editing of a large DNA virus: African Swine Fever Virus. Bio-protocol. 8(16). https://doi.org/10.21769/BioProtoc.2978.
The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host protein IL-1ß Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Ramirez, E., Vuono, E., Berggren, K., Alfano, M., Carey, L.B., Richt, J.A., Risatti, G.R., Gladue, D.P. 2018. The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host protein IL-1ß. Virus Research. 249:116-123. https://doi.org/10.1016/j.virusres.2018.03.017.
ICTV Virus taxonomy profile: Asfarviridae Reprint Icon ()
Alonso, C., Borca, M.V., Dixon, L., Revilla, Y., Rodriguez, F., Escribano, J.M. 2018. ICTV Virus taxonomy profile: Asfarviridae. Journal of General Virology. 99:613-614. https://doi.org/10.1099/jgv.0.001049.
CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Holinka-Patterson, L.G., Berggren, K., Gladue, D.P. 2018. CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses. Scientific Reports. 8(3154). https://doi.org/10.1038/s41598-018-21575-8.
Systemic antibodies administered by passive immunization prevent generalization of the infection by foot-and-mouth disease virus in cattle after oronasal challenge Reprint Icon - (Peer Reviewed Journal)
Barrionuevo, F., Di Giacomo, S., Bucafusco, D., Ayude, A., Schammas, J., Miraglia, M.C., Capozzo, A., Borca, M.V., Perez-Filgueira, M. 2018. Systemic antibodies administered by passive immunization prevent generalization of the infection by foot-and-mouth disease virus in cattle after oronasal challenge. Virology. 518:143-151. https://doi.org/10.1016/j.virol.2018.02.012.
A partial deletion within foot-and-mouth disease virus non-structural protein 3A causes clinical attenuation in cattle but does not prevent subclinical infection Reprint Icon - (Peer Reviewed Journal)
Stenfeldt, C., Arzt, J., Pacheco, J., Gladue, D.P., Smoliga, G.R., Silva, E., Rodriguez, L.L., Borca, M.V. 2018. A partial deletion within foot-and-mouth disease virus non-structural protein 3A causes clinical attenuation in cattle but does not prevent subclinical infection. Virology. 516:115-126. https://doi.org/10.1016/j.virol.2018.01.008.
Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses Reprint Icon - (Peer Reviewed Journal)
Largo, E., Gladue, D.P., Aguilella, V.M., Alcaraz, A., Borca, M.V., Nieva, J.L. 2018. Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses. Biochimica et Biophysica Acta. 1860:1015-1021. https://doi.org/10.1016/j.bbamem.2018.01.012.
Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G - (Peer Reviewed Journal)
Holinka-Patterson, L.G., O'Donnell, V., Risatti, G., Azzinaro, P.A., Arzt, J., Stenfeldt, C., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2017. Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G. PLoS One. doi: 10.1371/journal.pone.0177433.
Development of a fluorescent ASFV strain that retains the ability to cause disease in swine - (Peer Reviewed Journal)
Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Sanford, B., Azzinaro, P.A., Risatti, G., Gladue, D.P. 2017. Development of a fluorescent ASFV strain that retains the ability to cause disease in swine. Nature Scientific Reports. 7:46747. doi: 10.1038/srep46747.
Simultaneous deletion of the 9GL and UK genes from the African swine fever virus Georgia 2007 isolate results in virus attenuation and may be a potential virus vaccine strain - (Peer Reviewed Journal)
O'Donnell, V., Risatti, G.R., Holinka-Patterson, L.G., Krug, P.W., Carlson, J., Velazquez-Salinas, L., Azzinaro, P., Gladue, D.P., Borca, M.V. 2017. Simultaneous deletion of the 9GL and UK genes from the African swine fever virus Georgia 2007 isolate results in virus attenuation and may be a potential virus vaccine strain. Journal of Virology. 91(1):e01760-16.
Association of the host immune response with protection using a live attenuated African swine fever virus model Reprint Icon - (Peer Reviewed Journal)
Carlson, J., O'Donnell, V.K., Alfano, M., Velazquez-Salinas, L., Holinka-Patterson, L.G., Krug, P.W., Gladue, D.P., Higgs, S., Borca, M.V. 2016. Association of the host immune response with protection using a live attenuated African swine fever virus model . Viruses. 8(10):E291. https://doi.org/10.3390/v8100291.
Association of the host immune response with protection using a live attenuated African swine fever virus model - (Peer Reviewed Journal)
Carlson, J., O'Donnell, V., Alsfonso, M., Velazquez Salinas, L., Holinka-Patterson, L.G., Krug, P.W., Gladue, D.P., Higgs, S., Borca, M.V. 2016. Association of the host immune response with protection using a live attenuated african swine fever virus model. Viruses. 8(10):291. doi: 10.3390/v8100291.
Alteration of a second putative fusion peptide of structural glycoprotein E2 of Classical Swine Fever Virus alters virus replication and virulence in swine - (Peer Reviewed Journal)
Holinka-Patterson, L.G., Largo, E., Gladue, D.P., O'Donnell, V.K., Risatti, G.R., Nieva, J.L., Borca, M.V. 2016. Alteration of a second putative fusion peptide of structural glycoprotein E2 of Classical Swine Fever Virus alters virus replication and virulence in swine. Journal of Virology. 90:10299-10308. doi: 10.1128/JVI.01530-16.
The Ep152R ORF of African Swine Fever Virus strain Georgia encodes for an essential gene that interacts with host protein BAG6 Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Rai, D., Sanford, B., Alfano, M., Carlson, J., Azzinaro, P., Alonso, C., Gladue, D.P. 2016. The Ep152R ORF of African Swine Fever Virus strain Georgia encodes for an essential gene that interacts with host protein BAG6. Virus Research. 223:181-189. doi: 10.1016/j.virusres.2016.07.013.
African Swine Fever Virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes in highly attenuated in swine but does not confer protection against parental virus challenge - (Peer Reviewed Journal)
O'Donnell, V.K., Holinka-Patterson, L.G., Gladue, D.P., Sanford, B., Krug, P.W., Carlson, J., Pacheco, J., Reese, B., Risatti, G.R., Borca, M.V. 2016. African Swine Fever Virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes in highly attenuated in swine but does not confer protection against parental virus challenge. Virology. 221:8-14. doi: 10.1016/j.virusres.2016.05.014.
Recoding structural glycoprotein E2 in classical swine fever virus (CSFV) produces complete virus attenuation in swine and protects infected animals against disease - (Peer Reviewed Journal)
Velazquez-Salinas, L., Risatti, G.R., Holinka-Patterson, L.G., O'Donnell, V.K., Carlson, J., Alfano, M., Rodriguez, L.L., Carrillo, C., Gladue, D.P., Borca, M.V. 2016. Recoding classical swine fever virus (CSFV) structural glycoprotein E2 produces complete virus attenuation in swine and protects infected animals against disease. Virology. 494:178-189. doi: 10.1016/j.virol.2016.4.007.
Viral hemorrhagic fevers of animals caused by DNA viruses ()
Borca, M.V., Gay, C.G., Risatti, G.R., O'Toole, D., Li, N., Kuhn, J.H., Lewis, C.E., Loiacono, C.M., White, D. 2016. Viral hemorrhagic fevers of animals caused by DNA viruses. Book Chapter. Borca. 2015. Global Virology I: Identifying and Invetsigating Viral Diseases. Springer Science, NY.
Viral hemorrhagic fevers of animals caused by positive-stranded RNA viruses ()
Borca, M.V., Van Campen, H., Risatti, G.R., Kerr, P., Strive, T., Jhaling, P.B., Kuhn, J.H., Lewis, C.E., Loiacono, C.M., White, D. 2016. Viral hemorrhagic fevers of animals caused by positive-stranded RNA viruses. Book Chapter. Borca, M. V. Global Virology I: Springer Science, NY P 361-401.
Overview of Classical Swine Fever (Hog Cholera, Classical Swine fever) ()
Risatti, G.R., Borca, M.V. 2016. Overview of Classical Swine Fever (Hog Cholera, Classical Swine fever) . Book Chapter. Borca, M.V. The Merck Veterinary Manual. The Merck Publishing Group 2016.
Deletion of the thymidine kinase gene induces complete attenuation of the Georgia isolate of African swine fever virus - (Peer Reviewed Journal)
Sandford, B., Holinka-Patterson, L.G., O'Donnell, V., Krug, P.W., Carlson, J., Alfano, M., Carrillo, C., Wu, P., Lowe, A., Risatti, G.R., Gladue, D.P., Borca, M.V. 2015. Deletion of the thymidine kinase gene induces complete attenuation of the Georgia isolate of African swine fever virus. Virus Research. 213:165-171. doi: 10.1016/j.viruses.2015.12.002.
Systemic foot-and-mouth disease vaccination in cattle promotes specific antibody secreting cells at the respiratory tract and triggers local anamnestic-compatible responses upon aerosol infection Reprint Icon - (Peer Reviewed Journal)
Pega, J., Di Giacomo, S., Bucafusco, D., Schammas, J.M., Barrionuevo, F., Capozzo, A., Rodriguez, L.L., Borca, M.V., Perez-Filgueira, M. 2015. Systemic foot-and-mouth disease vaccination in cattle promotes specific antibody secreting cells at the respiratory tract and triggers local anamnestic-compatible responses upon aerosol infection. Journal of Virology. 89(18):9581-9590. doi: 10.1128/JVI.01082-15.
Deletion of African swine fever virus Georgia 2007 virulence-associated gene 9GL (B119L) leads to virus attenuation in swine at low doses while inducing an effective protection against homologous challenge - (Peer Reviewed Journal)
O'Donnell, V., Holinka-Patterson, L.G., Krug, P.W., Gladue, D.P., Carlson, J., Sanford, B.J., Alfano, M., Kramer, E., Lu, Z., Arzt, J., Reese, B., Carrillo, C., Risatti, G., Borca, M.V. 2015. Deletion of African swine fever virus Georgia 2007 virulence-associated gene 9GL (B119L) leads to virus attenuation in swine at low doses while inducing an effective protection against homologous challenge. Journal of Virology. 89(16):8556-8566. DOI: 10.1128/JVI.00969-15.
Treatment with interferon-alpha delays disease in swine infected with a highly virulent CSFV strain Reprint Icon - (Peer Reviewed Journal)
Fernandez-Sainz, I., Zhu, J.J., O'Donnell, V., Diaz San Segundo, F.C., Velazquez, L., De Los Santos, T.B., Borca, M.V. 2015. Treatment with interferon-alpha delays disease in swine infected with a highly virulent CSFV strain. Virology. 483:284-290. DOI: 10.1016/j.virol.2015.04.024.
Viral hemorrhagic fevers of animals caused by DNA viruses ()
Borca, M.V., Gay, C.G., Risatti, G., O'Toole, G., Li, H., Kuhn, J., Lewis, C., Loiacono, C., White, D.M. 2015. Viral hemorrhagic fevers of animals caused by DNA viruses. In:Shapshak, P., Sinnott, J.T., Somboonwit, C., Kuhn, J., editors. Global Virology-Identifying and Investigating Viral Diseases. New York, NY:Springer. p. 319-343.
African swine fever virus Georgia isolate harboring deletions of MGF360 and MGF505 genes is attenuated in swine and confers protection against challenge with the virulent parental virus Reprint Icon - (Peer Reviewed Journal)
O'Donnell, V., Holinka-Patterson, L.G., Gladue, D.P., Sanford, B.J., Krug, P.W., Lu, Z., Arzt, J., Reese, B., Carrillo, C., Risatti, G.R., Borca, M.V. 2015. African swine fever virus Georgia isolate harboring deletions of MGF360 and MGF505 genes is attenuated in swine and confers protection against challenge with the virulent parental virus. Journal of Virology. 89(11):6048-6056.
Progressive adaptation of a Georgian isolate of African swine fever virus to vero cells leads to a gradual attenuation of virulence in swine corresponding to major changes of the viral genome Reprint Icon - (Peer Reviewed Journal)
Krug, P.W., Holinka-Patterson, L.G., O'Donnell, V., Reese, B., Sanford, B.J., Fernandez-Sainz, I., Gladue, D.P., Arzt, J., Rodriguez, L.L., Risatti, G.R., Borca, M.V. 2014. Progressive adaptation of a Georgian isolate of African swine fever virus to vero cells leads to a gradual attenuation of virulence in swine corresponding to major changes of the viral genome. Journal of Virology. 89(4):2324-2332.
Virus-host interactions in persistently FMDV-infected cells derived from bovine pharynx - (Peer Reviewed Journal)
O'Donnell, V., Pacheco Tobin, J., Larocco, M.A., Gladue, D.P., Pauszek, S.J., Smoliga, G.R., Krug, P.W., Baxt, B., Borca, M.V., Rodriguez, L.L. 2014. Virus-host interactions in persistently FMDV-infected cells derived from bovine pharynx. Virology. 468-470C:185-196.
Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A - (Peer Reviewed Journal)
Stenfeldt, C., Pacheco Tobin, J., Borca, M.V., Rodriguez, L.L., Arzt, J. 2014. Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A. Acta Veterinaria Scandinavica. 56(1):42.
Interaction of structural core protein of Classical Swine Fever Virus with endoplasmic reticulum-associated degradation pathway protein OS9 - (Peer Reviewed Journal)
Gladue, D.P., O'Donnell, V., Fernandez-Sainz, I.J., Fletcher, P., Baker-Branstetter, R., Holinka-Patterson, L.G., Sanford, B.J., Carlson, J., Lu, Z., Borca, M.V. 2014. Interaction of structural core protein of Classical Swine Fever Virus with endoplasmic reticulum-associated degradation pathway protein OS9. Virology. 460-461:173-179. DOI: 10.1016/j.virol.2014.05.008.
Effect of specific amino acid substitutions in the putative fusion peptide of structural glycoprotein E2 on Classical Swine Fever Virus replication - (Peer Reviewed Journal)
Fernandez-Sainz, I.J., Largo, E., Gladue, D.P., Fletcher, P., O'Donnell, V., Holinka-Patterson, L.G., Carey, B., Lu, Z., Nieva, J.L., Borca, M.V. 2014. Effect of specific amino acid substitutions in the putative fusion peptide of structural glycoprotein E2 on Classical Swine Fever Virus replication. Virology. 456-457:121-130.
Interaction of foot-and-mouth disease virus non-structural protein 3A with host protein DCTN3 is important for viral virulence in cattle - (Peer Reviewed Journal)
Gladue, D.P., O'Donnell, V., Baker-Branstetter, R., Pacheco Tobin, J., Holinka-Patterson, L.G., Arzt, J., Pauszek, S.J., Fernandez-Sainz, I., Fletcher, P., Brocchi, E., Lu, Z., Rodriguez, L.L., Borca, M.V. 2014. Interaction of foot-and-mouth disease virus non-structural protein 3A with host protein DCTN3 is important for viral virulence in cattle. Journal of Virology. 88(5):2737-2747.
Interaction of CSFV E2 protein with swine host factors as detected by yeast two-hybrid system - (Peer Reviewed Journal)
Gladue, D.P., Baker-Branstetter, R., Holinka-Patterson, L.G., Fernandez-Sainz, I.J., O'Donnell, V., Fletcher, P., Lu, Z., Borca, M.V. 2014. Interaction of CSFV E2 protein with swine host factors as detected by yeast two-hybrid system . PLoS One. 9(1):e68532.
Pore-forming activity of pestivirus p7 in a minimal model system supports genus-specific viroporin function - (Peer Reviewed Journal)
Largo, E., Gladue, D.P., Huarte, N., Borca, M.V., Nieva, J.L. 2013. Pore-forming activity of pestivirus p7 in a minimal model system supports genus-specific viroporin function. Antiviral Research. 101:30-36.
The pathogenesis of highly virulent African Swine Fever virus in domestic pigs exposed via intraoropharyngeal, intranasopharyngeal, and intramuscular inoculation, and by direct contact with infected pigs Reprint Icon - (Peer Reviewed Journal)
Howey, E.B., O'Donnell, V., De Carvalho Ferreira, H.C., Borca, M.V., Arzt, J. 2013. The pathogenesis of highly virulent African Swine Fever virus in domestic pigs exposed via intraoropharyngeal, intranasopharyngeal, and intramuscular inoculation, and by direct contact with infected pigs. Virus Research. 178(2):328-339.
A partial deletion in non-structural protein 3A can attenuate foot-and-mouth disease virus in cattle Reprint Icon - (Peer Reviewed Journal)
Pacheco Tobin, J., Gladue, D.P., Holinka-Patterson, L.G., Arzt, J., Bishop, E.A., Smoliga, G.R., Pauszek, S.J., Bracht, A.J., O'Donnell, V., Fernandez-Sainz, I., Fletcher, P., Piccone, M.E., Rodriguez, L.L., Borca, M.V. 2013. A partial deletion in non-structural protein 3A can attenuate foot-and-mouth disease virus in cattle. Virology. 446(1-2):260-267.
Heterogeneity in the antibody response to foot-and-mouth disease primo-vaccinated calves - (Peer Reviewed Journal)
Di Giacomo, S., Brito, B.P., Perez, A.M., Bucafusco, D., Pega, J., Rodriguez, L.L., Borca, M.V., Perez-Filgueira, M. 2013. Heterogeneity in the antibody response to foot-and-mouth disease primo-vaccinated calves. Transboundary and Emerging Diseases. DOI:10.1111/tbed.12130.
Foot-and-mouth disease virus modulates cellular vimentin for virus survival Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., O'Donnell, V., Baker-Branstetter, R., Holinka-Patterson, L.G., Pacheco Tobin, J., Fernandez Sainz, I.J., Lu, Z., Brocchi, E., Piccone, M.E., Fletcher, P., Ambroggio, X., Rodriguez, L.L., Borca, M.V. 2013. Foot-and-mouth disease virus modulates cellular vimentin for virus survival. Journal of Virology. 87(12):6794-6803.
Early adaptive immune responses in the respiratory tract of foot and mouth disease-infected cattle Reprint Icon - (Peer Reviewed Journal)
Pega, J., Bucafusco, D., Di Giacomo, S., Malacari, D., Capozzo, A., Arzt, J., Perez-Beascoechea, C., Maradei, E., Rodriguez, L.L., Borca, M.V., Perez-Filgueira, M. 2012. Early adaptive immune responses in the respiratory tract of foot and mouth disease-infected cattle. Journal of Virology. 87(5):2489-2495.
Foot and mouth disease virus non structural protein 2C interacts with Beclin1 modulating virus replication Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., O'Donnell, V., Baker-Branstetter, R., Pacheco Tobin, J., Holinka-Patterson, L.G., Fernandez Sainz, I.J., Lu, Z., Brocchi, E., Baxt, B., Piconne, M.E., Rodriguez, L.L., Borca, M.V. 2012. Foot and mouth disease virus non structural protein 2C interacts with Beclin1 modulating virus replication. Journal of Virology. 86(22):12080-12090. doi:10.1128/JVI.01610-12.
Classical Swine Fever Virus p7 protein is a viroporin involved in virulence in swine Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., Holinka-Patterson, L.G., Largo, E., Fernandez Sainz, I.J., O'Donnell, V., Baker-Branstetter, R., Lu, Z., Ambroggio, X., Risatti, G.R., Nieva, J.L., Borca, M.V. 2012. Classical Swine Fever Virus p7 protein is a viroporin involved in virulence in swine. Journal of Virology. 86(12):6778-6791.
Role of the Arg56 of the structural protein VP3 of foot-and-mouth disease virus (FMDV) 01 campos in virus virulence Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Pacheco Tobin, J., Holinka-Patterson, L.G., Hartwig, E.J., Carrillo, C., Garriga, D., Kramer, E., Rodriguez, L.L., Piccone, M.E. 2012. Role of the Arg56 of the structural protein VP3 of foot-and-mouth disease virus (FMDV) 01 campos in virus virulence. Virology. (422):37-45.
Effects of glycosylation on antigenicity and immunogenicity of classical swine fever virus envelope proteins Reprint Icon - (Peer Reviewed Journal)
Gavrilov, B.K., Rogers, K., Fernandez Sainz, I.J., Holinka-Patterson, L.G., Borca, M.V., Risatti, G.R. 2011. Effects of glycosylation on antigenicity and immunogenicity of classical swine fever virus envelope proteins. Virology. 420:135-145.
Interaction between core protein of classical swine fever virus with cellular IQGAP1 proetin appears essential for virulence in swine - (Peer Reviewed Journal)
Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., O'Donnell, V.K., Vepkhvadze, N., Lu, Z., Risatti, G.R., Borca, M.V. 2011. Interaction between core protein of classical swine fever virus with cellular IQGAP1 proetin appears essential for virulence in swine. Virology. 412:68-74.
Identification of an NTPase motif in classical swine fever virus NS4B protein - (Peer Reviewed Journal)
Gladue, D.P., Gavrilov, B.K., Holinka-Patterson, L.G., Fernandez-Sainz, I.J., Vepkhvadze, N.G., Rogers, K., O'Donnell, V., Borca, M.V. 2011. Identification of an NTPase motif in classical swine fever virus NS4B protein. Virology. 411(1):41-49.
Foot-and-mouth disease virus utilizes an autophagic pathway during viral replication - (Peer Reviewed Journal)
O'Donnell, V., Pacheco Tobin, J., Larocco, M.A., Burrage, T., Jackson, W., Rodriguez, L.L., Borca, M.V., Baxt, B. 2010. Foot-and-mouth disease virus utilizes an autophagic pathway during viral replication. Virology. 410:142-150.
Substitution of specific cysteine residues in E1 glycoprotein of classical swine fever virus strain Brescia affects formation of E1-E2 heterodimers and alters virulence in swine - (Peer Reviewed Journal)
Fernandez-Sainz, I.J., Holinka-Patterson, L.G., Gladue, D.P., O'Donnell, V., Lu, Z., Gavrilov, B.K., Risatti, G.R., Borca, M.V. 2011. Substitution of specific cysteine residues in E1 glycoprotein of classical swine fever virus strain Brescia affects formation of E1-E2 heterodimers and alters virulence in swine. Journal of Virology. 407:129-136.
Effects of the interactions of classical swine fever virus core protein with proteins of SUMOylation pathway on virulence in swine - (Peer Reviewed Journal)
Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Saniz, I., Prarat, M.V., O'Donnell, V.K., Vepkhvadze, N., Lu, Z., Rogers, K., Risatti, G.R., Borca, M.V. 2010. Effects of the interactions of classical swine fever virus core protein with proteins of SUMOylation pathway on virulence in swine. Virology. 407:129-136.
Domain disruptions of individual 3B proteins for foot-and-mouth disease virus do not alter growth in cell culture nor virulence in cattle - (Peer Reviewed Journal)
Pacheco Tobin, J., Piccone, M.E., Rieder, A.E., Pauszek, S.J., Borca, M.V., Rodriguez, L.L. 2010. Domain disruptions of individual 3B proteins for foot-and-mouth disease virus do not alter growth in cell culture nor virulence in cattle. Virology. DOI: 10.1016/j.virol.2010.05.036.
Mutations in the classical swine fever virus NS4B protein affects virulence in swine - (Abstract Only)
Fernandez Sainz, I., Gladue, D.P., Holinka-Patterson, L.G., O'Donnell, V.K., Gudmundsdottir, I., Prarat, M.V., Patch, J.R., Golde, W.T., Zhiqiang, L., Risatti, G.R., Zhu, J.J., Borca, M.V. 2009. Mutations in the classical swine fever virus NS4B protein affects virulence in swine. Proceedings. P. 143.
Mutations in classical swine fever virus NS4B affect virulence in swine - (Peer Reviewed Journal)
Fernandez-Sainz, I., Gladue, D.P., Holinka-Patterson, L.G., O'Donnell, V.K., Gudmundsdottir, I., Prarat, M.V., Patch, J.R., Golde, W.T., Lu, Z., Risatti, G.R., Zhu, J.J., Borca, M.V. 2010. Mutations in classical swine fever virus NS4B affect virulence in swine. Journal of Virology. 84(3):1536-1549.
The region between the two polyprotein initiation codons of foot-and-mouth disease virus is critical for virulence in cattle - (Peer Reviewed Journal)
Piccone, M.E., Pacheco Tobin, J., Pauszek, S.J., Borca, M.V., Rieder, A.E., Kramer Jr, E.V., Zhu, J.J., Rodriguez, L.L. 2010. The region between the two polyprotein initiation codons of foot-and-mouth disease virus is critical for virulence in cattle. Virology. 396:152-159.
Sumoylation of the Core Protein in Classical Swine Fever Virus is Essential for Virulence in swine - (Abstract Only)
Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., Lu, Z., Borca, M.V. 2009. Sumoylation of the Core Protein in Classical Swine Fever Virus is Essential for Virulence in swine. American Society for Virology Meeting. Paper No. W49-5: P. 213.
Alteration of the N-linked Glycosylation Condition of E1 Glycoprotein of Classical Swine Fever Virus Strain Brescia Alters Virulence in Swine - (Peer Reviewed Journal)
Fernandez-Sainz, I.J., Holinka-Patterson, L.G., Gavrilov, B.K., Prarat, M.V., Gladue, D.P., Lu, Z., Jia, W., Risatti, G.R., Borca, M.V. 2009. Alteration of the N-linked Glycosylation Condition of E1 Glycoprotein of Classical Swine Fever Virus Strain Brescia Alters Virulence in Swine. Virology. 386:210-216.
Development of a live attenuated antigenic marker classical swine fever vaccine - (Peer Reviewed Journal)
Holinka-Patterson, L.G., Fernandez-Sainz, I., O'Donnell, V., Prarat, M.V., Gladue, D.P., Lu, Z., Risatti, G., Borca, M.V. 2008. Development of a live attenuated antigenic marker classical swine fever vaccine. Virology. 384:106-113.
Patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray - (Peer Reviewed Journal)
Gladue, D.P., Zhu, J.J., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., O'Donnell, V.K., Borca, M.V. 2010. Patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray. Virology. 138(1-2):89-96.
Patterns of Cellular Gene Expression in Swine Macrophages Infected with Highly Virulent Classical Swine Fever Virus Strain Brescia - (Peer Reviewed Journal)
Borca, M.V., Gudmundsdottir, I., Fernandez-Sainz, I.J., Holinka-Patterson, L.G., Risatti, G.R. 2008. Patterns of Cellular Gene Expression in Swine Macrophages Infected with Highly Virulent Classical Swine Fever Virus Strain Brescia. Virus Research (138): 89-96
Role of the Region Located Between the Two AUG Initiation Codons in the Pathogenesis of Foot-and-Mouth Disease Virus (FMDV) - (Abstract Only)
Piccone, M.E., Pacheco Tobin, J., Pauszek, S.J., Borca, M.V., Rieder, A.E., Rodriguez, L.L. 2008. Role of the Region Located Between the Two AUG Initiation Codons in the Pathogenesis of Foot-and-Mouth Disease Virus (FMDV). European Study Group on the Molecular Biology of Picornaviruses. p. 199.
Removal of a N-linked Glycosylation Site on the Classical Swine Fever Virus Strain Brescia E(rns) Glycoprotein Affects Virulence in Swine - (Peer Reviewed Journal)
Fernandez-Sainz, I., Holinka, L.G., Lu, Z., Risatti, G.R., Borca, M.V. 2007. Removal of a N-linked Glycosylation Site on the Classical Swine Fever Virus Strain Brescia E(rns) Glycoprotein Affects Virulence in Swine. Virology. 370:122-129.
Sheeppox virus kelch-like gene SPPV-019 affects virus virulence - (Peer Reviewed Journal)
Balinsky, C.A., Delhon, G., Afonso, C.L., Risatti, G.R., Borca, M.V., French, R.A., Tulman, E.R., Geary, S.J., Rock, D.L. 2007. Sheeppox virus kelch-like gene SPPV-019 affects virus virulence. Journal of Virology. 81(20):11392-11401.
Genetic and Phenotypic Variation of FMDV During Serial Passages in a Natural Host - (Peer Reviewed Journal)
Carrillo, C., Lu, Z., Borca, M.V., Vagnozzi, A., Kutish, G.F., Rock, D.L. 2007. Genetic and Phenotypic Variation of FMDV During Serial Passages in a Natural Host. Journal of Virology. 81(20):11341-11351. DOI: 10-1128/jvi.00930-07.
Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages - (Peer Reviewed Journal)
Zaffuto, K.M., Piccone, M.E., Burrage, T.G., Risatti, G.R., Borca, M.V., Holinka, L.G., Rock, D.L., Afonso, C.L. 2007. Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages. Journal of General Virology. 88:3007-3012.
Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus are Responsible for Viral Attenuation in Swine - (Abstract Only)
Risatti, G.R., Holinka, L.G., Fernanez-Sainz, I., Carrillo, C., Kutish, G., Lu, Z., Zhu, J., Rock, D.L., Borca, M.V., 2007, Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus are Responsible for Viral Attenuation in Swine. Positive Strand RNA Virus International Conference Proceedings. P2-K27, p.116
N-linked Glycosylation of Classical Swine Fever Virus Strain Brescia Erns Glycoprotein Alters Virulence in Swine - (Abstract Only)
Fernandez-Sainz, I., Holinka, L.G., Lu, Z., Risatti, G.R., Borca, M.V. 2007. N-linked Glycosylation of Classical Swine Fever Virus Strain Brescia Erns Glycoprotein Alters Virulence in Swine. Eighth International Symposium on Positive Strand RNA Virus, P2-K26, P.116
Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine - (Peer Reviewed Journal)
Risatti, G., Holinka, L.G., Fernandez-Sainz, I., Carrillo, C., Kutish, G.F., Lu, Z., Zhu, J., Rock, D.L., Borca, M.V. 2007. Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine. Virology. 364 (2): 371-82.
N-Linked Glycosylation Status Of Classical Swine Fever Virus Strain Brescia E2 Glycoprotein Influences Virulence In Swine - (Abstract Only)
Risatti, G., Holinka, L.G., Fernandez-Sainz, I., Carrillo, C., Lu, Z., Borca, M.V. 2006. N-linked glycosylation status of classical swine fever virus strain brescia e2 glycoprotein influences virulence in swine. Conference of Research Workers in Animal Disease (CRWAD) annual meeting, Chicago, IL, December 3-5, 2006, page 200.
Mutations in the Carboxi Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine - (Abstract Only)
Risatti, G.R., Holinka, L.G., Fernandez-Sainz, I., Carillo, C., Kutish, G.F., Lu, Z., Tulman, E.R., Rock, D.L., Borca, M.V. Mutations in the Carboxi Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine. Conference of Research Workers in Animal Disease (CRWAD) annual meeting, Chicago, IL, December 3-5, 2006, page 138.
Identification of a Novel Virulence Determinant Within the E2 Structural Glycoprotein of Classical Swine Fever Virus - (Abstract Only)
Risatti, G.R., Holinka, L.G., Carillo, C., Kutish, G.F., Lu, Z., Tulman, E.R., Fernandez-Sainz, I., Borca, M.V. 2006. Identification of a Novel Virulence Determinant Within the E2 Structural Glycoprotein of Classical Swine Fever Virus. Conference of Research Workers in Animal Disease (CRWAD) annual meeting, Chicago, IL, December 3-5, 2006, page 201.
N-LINKED GLYCOSYLATION STATUS OF CLASSICAL SWINE FEVER VIRUS STRAIN BRECIA E2 GLYCOPROTEIN INFLUENCES VIRULENCE IN SWINE - (Peer Reviewed Journal)
Rissatti, G., Holinka, L.G., Fernandez-Sainz, I., Carrillo, C., Lu, Z., Borca, M.V. 2007. N-linked glycosylation status of classical swine fever virus strain brecia e2 glycoprotein influences virulence in swine. Journal of Virology. Vol. 81, No.2, P. 924-933.
IDENTIFICATION OF A NOVEL VIRULENCE DETERMINAT WITHIN THE E2 STRUCTURAL GLYCOPROTEIN OF CLASSICAL SWINE FEVER - (Peer Reviewed Journal)
Risatti, G., Holinka, L.G., Carrillo, C., Kutish, G.F., Lu, Z., Fernandez-Sainz, I., Borca, M.V. 2006. Identification of a novel virulence determinat within the e2 structural glycoprotein of classical swine fever. Virology, 11/10/2006, Vol 355, Issue i, pages 94-101.
MUTATION OF E1 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Peer Reviewed Journal)
Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Tulman, E.R., French, R.A., Sur, J.H., Rock, D.L., Borca, M.V. 2005 Mutation of E1 Glycoprotein of Classical Swine Fever Virus Affects Viral Virulence in Swine. Journal of Virology 343: 116-127.
CHIMERIC CLASSICAL SWINE FEVER VIRUS CONTAINING THE E2 GENE FROM A VACCINE STRAIN IN THE GENETIC BACKGROUND OF STRAIN BRESCIA INDUCES SOLID EARLY PROTECTION IN SWINE - (Abstract Only)
Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Rock, D.L., Borca, M.V. 2005. Chimeric Classical Swine Fever Virus Containing the E2 Gene from a Vaccine Strain in the Genetic Background of Strain Brescia Induces Solid Early Protection in Swine. 6th Pestivirus Symposium, Thun, Switzerland.Page 89, P5-10
GENETIC AND PHENOTYPIC VARIATION OF FMDV DURING SERIAL PASSAGES IN PIGS - (Abstract Only)
Carrillo, C., Lu, Z., Tulman, E., Borca, M.V., Vagnozzi, A., Kutish, G.F., Rock, D.L. 2005. Genetic and Phenotypic Variation of FMDV During Serial Passages in Pigs. European Study Group on the Molecular Biology of Picornaviruses. P. E06.
NOVEL TOOLS TO PREVENT AND ERADICATE FOREIGN ANIMAL DISEASES - (Abstract Only)
Rodriguez, L.L., Baxt, B., Rieder, A.E., Golde, W.T., Borca, M.V., Grubman, M.J. 2005. Novel tools to prevent and eradicate foreign animal diseases. American Society for Microbiology Biodefense Meeting. p 41.
PRECLINICAL DIAGNOSIS OF AFRICAN SWINE FEVER IN CONTACT EXPOSED SWINE BY A PORTABLE REAL-TIME PCR ASSAY - (Proceedings)
Zsak, L., Borca, M.V., Risatti, G.R., French, R.A., Lu, Z., Kutish, G.F., Neilan, J.G., Callahan, J.D., Nelson, W.M., Rock, D.L. 2004. Preclinical diagnosis of african swine fever in contact exposed swine by a portable real-time pcr assay [abstract]. Conference Research Workers Disease Meeting. p. 87.
THE E2 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Peer Reviewed Journal)
Risatti, G.R., Borca, M.V., Kutish, G.F., Lu, Z., Holinka, L.G., French, R., Tulman, E., Rock, D.L. 2005 The E2 Glycoprotein of Classical Swine Fever Virus Affects Viral Virulence in Swine. Journal of Virology.79: 3787-3796.
A 19MER PEPTIDE INSERTION IN THE E1 GLYCOPROTEIN OF CLASSICAL SWINE FEVER FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Proceedings)
Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Sur, J.H., Rock, D.L., Borca, M.V. 2004. A 19mer Peptide Insertion in the E1 Glycoprotein of Classical Swine Fever Virus Affects Viral Virulence in Swine. Conference of Research Worksers in Animal Disese Meeting. P.195
THE E2 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS IS A VIRULENCE DETERMINANT IN SWINE - (Proceedings)
Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Tulman, E., Rock, D.L., Borca, M.V. 2004. The E2 Glycoprotein of Classical Swine Fever Virus is a Virulence Determinant in Swine. Conference of Research Worksers in Animal Disese Meeting. P. 196.
DIAGNOSTIC EVALUATION OF A PORTABLE REAL-TIME REVERSE TRANSCRIPTASE PCR ASSAY FOR THE DETECTION OF CLASSICAL SWINE FEVER. - (Proceedings)
Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Callahan, J.D., Nelson, W.N., Tio, B.E., Borca, M.V. 2004. Diagnostic Evaluation of a Portable Real-Time Reverse Transcriptase PCR Assay for the Detection of Classical Swine Fever. Conference of Research Worksers in Animal Disese Meeting. P 194.
DIAGNOSTIC EVALUATION OF A PORTABLE REAL-TIME REVERSE TRANSCRIPTASE PCR ASSAY FOR THE DETECTION OF CLASSICAL SWINE FEVER - (Peer Reviewed Journal)
Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Callahan, J.D., Nelson, W.M., Brea, T.E., Borca, M.V. 2005. Diagnostic Evaluation of a Portable Real-Time Reverse transcriptase PCR Assay for the Detection of Classical Swine Fever. Journal of Clinical Microbiology. 43 (1): 468-471.
PRECLINICAL DIAGNOSIS OF AFRICAN SWINE FEVER IN CONTACT EXPOSED SWINE BY A PORTABLE REAL-TIME PCR ASSAY - (Peer Reviewed Journal)
PRECLINICAL DIAGNOSIS OF AFRICAN SWINE FEVER IN CONTACT EXPOSED SWINE BY A PORTABLE REAL-TIME PCR ASSAY - (Peer Reviewed Journal)
Zsak, L., Borca, M.V., Risatti, G.R., French, R., Lu, Z., Kutish, G.F., Neilan, J.G., Callahan, J.D., Nelson, W.M., Rock, D.L. 2005. Preclinical Diagnosis of African Swine Fever in Contact Exposed Swine by a Portable Real-time PCR Assay. Journal of Clinical Microbiology. 43 (1):112-119.
DEVELOPMENT OF TRANSGENIC ALFALFA PLANTS CONTAINING THE FOOT AND MOUTH DISEASE VIRUS STRUCTURAL POLYPROTEIN GENE P1 AND ITS UTILIZATION AS AN EXPERIMENTAL IMMUNOGEN - (Peer Reviewed Journal)
Dus Santos, M., Carrillo, C., Ardila, F., Rios, R., Franzone, M., Piccone, M.E., Wigdorovitz, A., Borca, M.V. 2005. Development of Transgenic Alfalfa Plants Containing the Foot-and-Mouth Disease Virus Structural Polyprotein Gene p1 and Its Utilization as an Experimental Immunogen. Vaccine.23(15): 1838-43.
PRESERVED ANTIGENICITY OF HIV-1 P24 PRODUCED AND PURIFIED IN HIGH YIELDS FROM PLANTS INOCULATED WITH TOBACCO MOSAIC VIRUS (TMV)-DERIVED VECTOR - (Peer Reviewed Journal)
Perez, D., Brayfield, B., Phiri, S., Borca, M.V., Woods, C., Morris, T. 2004. Preserved antigenicity of hiv-1 p24 produced and purified in high yields from plants inoculated with tobacco mosaic virus (tmv)-derived vector.. Journal of Virological Methods. 121 (2004) 201-208.
PASSIVE PROTECTION TO BOVINE ROTAVIRUS (BRV) INFECTION INDUCED BY A BRV VP8* PRODUCED IN PLANTS USING A TMV-BASED VECTOR - (Peer Reviewed Journal)
Perez Filgueira, D., Mozgovoj, M., Wigdorovitz, A., Dus Santos, M., Parreno, V., Trono, K., Fernandez, F., Carrillo, C., Badiuk, L., Morris, T., Borca, M.V. 2004. Passive protection to bovine rotavirus (brv) infection induced by a brv vp8* produced in plants using a tmv-based vector.. Archives of Virology. 149:2337-2348.
A 19MER PEPTIDE INSERTION IN THE E1 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Proceedings)
Borca, M.V., Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Sur, J., Rock, D.L. 2004. A 19mer peptide insertion in the e1 glycoprotein of classical swine fever virus affects viral virulence in swine [abstract]. Positive Strand RNA Virus International Conference Proceedings. p. 109.
THE E2 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Proceedings)
Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Borca, M.V., Rock, D.L. 2004. The e2 glycoprotein of classical swine fever virus affects viral virulence in swine [abstract]. Positive Strand RNA Virus International Conference Proceedings. P. 109.
PROTECTIVE LACTOGENIC IMMUNITY CONFERRED BY AN EDIBLE PEPTIDE VACCINE TO BOVINE ROTAVIRUS PRODUCED IN TRANSGENIC PLANTS - (Peer Reviewed Journal)
Wigdorovitz, A., Mozgovoj, M., Dus Santos, M.J., Parreno, V., Perez-Filgueira, D.M., Trono, K.G., Rios, R.D., Carrillo, C., Escribano, J.M., Borca, M.V. 2004. Protective lactogenic immunity conferred by an edible peptide vaccine to bovine rotavirus produced in transgenic plants. Journal of General Virology. 85:1825-1832.
BOVINE HERPES VIRUS G-D PROTEIN PRODUCED IN PLANTS USING A RECOMBINANT TOBACCO MOSAIC VIRUS (TMV) VECTOR POSSESSES AUTHENTIC ANTIGENICITY - (Peer Reviewed Journal)
Perez Filgueira, D.M., Zamorano, P.I., Dominguez, M.G., Taboga, O., Del Medico Zajac, M.P., Puntel, M., Romera, S.A., Morris, T.J., Borca, M.V., Sadir, A.M. 2003. Bovine herpes virus g-d protein produced in plants using a recombinant tobacco mosaic virus (tmv) vector possesses authentic antigenicity. Vaccine. 21(27-30):4201-4209.
RAPID DETECTION OF CLASSICAL SWINE FEVER VIRUS BY A PORTABLE REAL-TIME REVERSE TRANSCRIPTASE PCR ASSAY - (Peer Reviewed Journal)
Rapid detection of classical swine fever virus by a portable real time reverse transcriptase-PCR asssay - (Peer Reviewed Journal)
Risatti, G.R., Callahan, J.D., Nelson, W.M., Borca, M.V. 2003. Rapid detection of classical swine fever virus by a portable real time reverse transcriptase-PCR asssay. Journal of Clinical Microbiology. 41(1):500-505. doi: 10.1128/jcm.41.1.500-505.2003.
ARS News Articles