Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 9/30/2004
Publication Date: 11/14/2004
Citation: Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Callahan, J.D., Nelson, W.N., Tio, B.E., Borca, M.V. 2004. Diagnostic Evaluation of a Portable Real-Time Reverse Transcriptase PCR Assay for the Detection of Classical Swine Fever. Conference of Research Worksers in Animal Disese Meeting. P 194.
Technical Abstract: A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase PCR for classical swine fever virus (CSFV) was evaluated for diagnostic sensitivity and specificity using clinical samples obtained from the Dominican Republic where the disease is enzootic. The test, using nasal swab samples taken from both symptomatic and asymptomatic animals, exceeded the diagnostic sensitivity of virus isolation (100% vs 72.4%, respectively) with little loss of specificity (98.9% vs 100%, respectively). At the herd level, 3 out of 4 infected farms were detected by virus isolation, while the CSF real time RT-PCR assay identified all four infected premises. This simple and accurate test permits rapid detection of CSFV in affected herds.