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Title: PRECLINICAL DIAGNOSIS OF AFRICAN SWINE FEVER IN CONTACT EXPOSED SWINE BY A PORTABLE REAL-TIME PCR ASSAY

Author
item Zsak, Laszlo
item Borca, Manuel
item Risatti, Guillermo
item FRENCH, RICHARD
item Lu, Zhiqiang
item Kutish, Gerald
item Neilan, John
item CALLAHAN, JOHNNY
item NELSON, WILLIAM
item Rock, Daniel

Submitted to: Conference Research Workers Disease Meeting
Publication Type: Proceedings
Publication Acceptance Date: 11/16/2004
Publication Date: 11/16/2004
Citation: Zsak, L., Borca, M.V., Risatti, G.R., French, R.A., Lu, Z., Kutish, G.F., Neilan, J.G., Callahan, J.D., Nelson, W.M., Rock, D.L. 2004. Preclinical diagnosis of african swine fever in contact exposed swine by a portable real-time pcr assay [abstract]. Conference Research Workers Disease Meeting. p. 87.

Interpretive Summary:

Technical Abstract: Clinical signs of African swine fever (ASF) are inapparent at early stages of infection and at later stages they resemble those of some other swine diseases. A rapid, preclinical diagnosis at the site of the suspected disease outbreak would be extremely useful for controlling ASF. To address this need, a fluorogenic probe hydrolysis (TaqMan®) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one step-single tube assay, which can be performed in two hours or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.