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Title: Virus-host interactions in persistently FMDV-infected cells derived from bovine pharynx

Author
item O'DONNELL, VIVIAN - University Of Connecticut
item Pacheco Tobin, Juan
item Larocco, Michael
item GLADUE, DOUGLAS - University Of Connecticut
item Pauszek, Steven
item Smoliga, George
item Krug, Peter
item BAXT, BARRY - Retired ARS Employee
item Borca, Manuel
item Rodriguez, Luis

Submitted to: Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/4/2014
Publication Date: 9/9/2014
Citation: O'Donnell, V., Pacheco Tobin, J., Larocco, M.A., Gladue, D.P., Pauszek, S.J., Smoliga, G.R., Krug, P.W., Baxt, B., Borca, M.V., Rodriguez, L.L. 2014. Virus-host interactions in persistently FMDV-infected cells derived from bovine pharynx. Virology. 468-470C:185-196.

Interpretive Summary: Foot-and-mouth disease is a highly contagious foreign animal disease of livestock, which results in large economic losses to countries where it occurs. The virus produces an acute infection in cloven-hoofed livestock which is characterized by vesicular lesions and, particularly in cattle, a persistent infection with asymptomatic low-level production of virus. In this study, we described the establishment of a persistently-FMDV infected primary cell culture. We examined the persistent virus recovered for mutations and growth. The development of persistently FMDV-infected bovine pharynx cultures can be used as an in vitro persistent model for FMDV persistence.

Technical Abstract: Foot-and-mouth disease virus (FMDV) produces a disease in cattle characterized by vesicular lesions and a persistent infection with asymptomatic low-level production of virus. Here we describe the establishment of a persistently infected primary cell culture derived from bovine pharynx tissue (PBPT) infected with FMDV serotype O1 Manisa, where surviving cells were serially passed until a persistently infected culture was generated. Characterization of the persistent virus demonstrated changes in its plaque size, ability to grow in different cell lines, loss of the ability to use integrins as receptors, and an adaptation to the PBPT cultures when compared with the parental virus. These results demonstrate for the first time the establishment of persistently infected PBPT cell cultures where a co-evolutionary process has taken place between the virus and host cells. This in vitro model for FMDV persistence may help further understanding of the molecular mechanisms of the cattle carrier state.