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Title: Dn10, a new gene conferring resistance to Russian wheat aphid biotype 2 in Iranian wheat landrace PI 682675

Author
item LI, GENQIAO - Oklahoma State University
item Xu, Xiangyan
item CARVER, BRETT - Oklahoma State University
item GUO, PEIGUO - Guangzhou University
item Puterka, Gary

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/18/2017
Publication Date: 5/3/2018
Citation: Li, G., Xu, X., Carver, B.F., Guo, P., Puterka, G.J. 2018. Dn10, a new gene conferring resistance to Russian wheat aphid biotype 2 in Iranian wheat landrace PI 682675. Crop Science. 58(3):1219-1225. https://doi.org/10.2135/cropsci2017.10.0649.
DOI: https://doi.org/10.2135/cropsci2017.10.0649

Interpretive Summary: Russian wheat aphid (RWA) is a serious pest of wheat and barley that causes heavy yield losses in many countries, and RWA biotype 2 (RWA2) is virulent to most RWA resistance genes. The objective of this study was to characterize a gene for resistance to RWA2 in Iranian landrace PI 682675, a single plant selection of PI 624151. F2:3 families and F3:4 families derived from cross PI 682675 × Zhouyou 6 were used to map the resistance gene. PI 682675 carries a dominant resistance gene, Dn10, flanked by SSR markers Xgwm437 and Xwmc488 in chromosome 7DL. Dn10 was mapped to the terminal region of chromosome 7DL, whereas Dn2401 and Dn616580 that also confer resistance to RWA2 were physically mapped to 7DS. Dn10 was located about 16.7 cM from Dn2401 and 20.9 cM from Dn626580. Dn10 is a new resistance gene that can be widely used in wheat breeding.

Technical Abstract: Russian wheat aphid (RWA) is a serious pest of wheat and barley that causes heavy yield losses in many countries, and RWA biotype 2 (RWA2) is virulent to most RWA resistance genes. The objective of this study was to characterize a gene for resistance to RWA2 in Iranian landrace PI 682675, a single plant selection from PI 624151. F2:3 families and F3:4 families derived from cross PI 682675 × Zhouyou 6 were used to map the resistance gene. PI 682675 carries a dominant resistance gene, Dn10, flanked by SSR markers Xgwm437 and Xwmc488 in chromosome 7DL. Physical mapping indicated that Dn10 resides in bin 7DL 0.1-0.77, whereas Dn2401 and Dn616580 that also confer resistance to RWA2 were physically mapped to 7DS. Allelism tests showed that Dn10 was located about 16.7 cM from Dn2401 and 20.9 cM from Dn626580. Dn10 is a new resistance gene that can be widely used in wheat breeding.