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United States Department of Agriculture

Agricultural Research Service

Related Topics

Gaylen A Uhlich
Molecular Characterization of Foodborne Pathogens Research
Microbiology

Phone: (215) 233-6740
Fax: (215) 233-6581
Room 2112.2

600 E MERMAID LN
ERRC
WYNDMOOR , PA 190388598

Projects
Shiga Toxin-Producing Escherichia coli in Biofilms and within Microbial Communities in Food
In-House Appropriated (D)
  Accession Number: 429656

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Whole-genome sequence of Escherichia coli serotype O157:H7 strain EDL932 (ATCC 43894) -
Uhlich, G.A., Paoli, G., Chen, C., Cottrell, B.J., Zhang, X., Yan, X. 2016. Whole-genome sequence of Escherichia coli serotype O157:H7 strain EDL932 (ATCC 43894). Genome Announcements. doi: 10.1128/genomeA.00647-16.
Stx1 prophage excision in Escherichia coli strain PA20 confers strong curli and biofilm formation by restoring native mlrA -
Uhlich, G.A., Chen, C., Cottrell, B.J., Yan, X., Hofmann, C.S., Nguyen, L.T. 2016. Stx1 prophage excision in Escherichia coli strain PA20 confers strong curli and biofilm formation by restoring native mlrA. FEMS Microbiology Letters. 10.1093/femsle/fnw123.
Multiple mechanisms responsible for strong Congo red-binding variants of Escherichia coli O157:H7 strains -
Chen, C., Nguyen, L.T., Cottrell, B.J., Irwin, P.L., Uhlich, G.A. 2015. Multiple mechanisms responsible for strong Congo red-binding variants of Escherichia coli O157:H7 strains. Pathogens and Disease. 74:123.
Genetically marked strains of Shiga toxin-producing O157:H7 and non-O157 Escherichia coli: Tools for detection and modelling Reprint Icon -
Paoli, G., Uhlich, G.A., Wijey, C. 2015. Genetically marked strains of Shiga toxin-producing O157:H7 and non-O157 Escherichia coli: Tools for detection and modelling. Journal of Food Protection. 78(5):888-901.
Growth media and temperature effects on biofilm formation by serotype O157:H7 and non-O157 Shiga toxin-producing Escherichia coli Reprint Icon -
Uhlich, G.A., Chen, C., Cottrell, B.J., Nguyen, L.T. 2014. Growth media and temperature effects on biofilm formation by serotype O157:H7 and non-O157 Shiga toxin-producing Escherichia coli. FEMS Microbiology Letters. 354:133-141.
Genetically marked strains of Shiga toxin-producing E. coli O157:H7 and non-O157 for detection and modeling -
Paoli, G., Uhlich, G.A., Wijey, C. 2015. Genetically marked strains of Shiga toxin-producing E. coli O157:H7 and non-O157 for detection and modeling. Meeting Abstract. meetingabstract.
Production and regulation of functional amyloid curli fimbriae by Shiga toxin-producing Escherichia coli -
Media composition and incubation temperature affect Congo red dye affinity of Shiga toxin-producing Escherichia coli -
Uhlich, G.A., Chen, C., Cottrell, B.J., Nguyen, L.T. 2014. Media composition and incubation temperature affect Congo red dye affinity of Shiga toxin-producing Escherichia coli. Meeting Abstract. MA.
Phenotypic and genotypic characterization of biofilm forming capability in non-O157 Shiga toxin-producing Escherichia coli strains -
Chen, C., Hofmann, C.S., Cottrell, B.J., Strobaugh Jr, T.P., Paoli, G., Nguyen, L.T., Yan, X., Uhlich, G.A. 2013. Phenotypic and genotypic characterization of biofilm forming capability in non-O157 Shiga toxin-producing Escherichia coli strains. PLoS One. 8(12):e84863.
Non-labeled quantitative proteomic comparison identifies differences in acid resistance between Escherichia coli O157:H7 curli production variants Reprint Icon -
Gunther, N.W., Liu, Y., Nunez, A., Paul, M., Uhlich, G.A. 2014. Non-labeled quantitative proteomic comparison identifies differences in acid resistance between Escherichia coli O157:H7 curli production variants. Foodborne Pathogens and Disease. 11:30-37.
Phage insertion in mlrA and variations in rpoS limit curli expression and biofilm formation in Escherichia coli serotype O157:H7 -
Uhlich, G.A., Chen, C., Cottrell, B.J., Hofmann, C.S., Dudley, E., Strobaugh Jr, T.P., Nguyen, L.T. 2013. Phage insertion in mlrA and variations in rpoS limit curli expression and biofilm formation in Escherichia coli serotype O157:H7. Microbiology. 159:1586-1596.
Prophage insertions in mlrA are not the major obstacle to biofilm formation in Escherichia coli O157:H7 -
Uhlich, G.A., Chen, C., Dudley, E.G., Hofmann, C.S., Cottrell, B.J. 2014. Prophage insertions in mlrA are not the major obstacle to biofilm formation in Escherichia coli O157:H7. Meeting Abstract. MA.
Mutations in the rpoS gene are the major limiting factor for biofilm formation in Escherichia coli serotype O157:H7 clinical isolates -
Chen, C., Uhlich, G.A., Strobaugh Jr, T.P., Cottrell, B.J., Dudley, E.G., Hofmann, C.S., Nguyen, L.T. 2013. Mutations in the rpoS gene are the major limiting factor for biofilm formation in Escherichia coli serotype O157:H7 clinical isolates. Meeting Abstract. MA.
Peroxide resistance in Escherichia coli serotype O157:H7 biofilms is regulated by both RpoS dependent and independent mechanisms -
Uhlich, G.A., Chen, C., Cottrell, B.J., Irwin, P.L., Phillips, J.G. 2012. Peroxide resistance in Escherichia coli serotype O157:H7 biofilms is regulated by both RpoS dependent and independent mechanisms. Microbiology. 158:2225-2234.
Understanding the role of the catalase/peroxide genes in H2O2 resistance of E. coli serotype O157:H7 biofilms -
Uhlich, G.A., Chen, C., Irwin, P.L. 2012. Understanding the role of the catalase/peroxide genes in H2O2 resistance of E. coli serotype O157:H7 biofilms [abstract]. 1:1.
Regulation of the csgD promoter by global regulators HN-S, IHF, and RpoS in E. coli O157:H7 isolates -
Chen, C., Uhlich, G.A. 2012. Regulation of the csgD promoter by global regulators HN-S, IHF, and RpoS in E. coli O157:H7 isolates [abstract]. Poster p-3-121.
A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's -
Irwin, P.L., Nguyen, L.T., Chen, C., Uhlich, G.A., Paoli, G. 2012. A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's. Analytical and Bioanalytical Chemistry. 402:2713-2725.
A cloning vector for creation of Escherichia coli lacZ translational fusions and generation of linear template for chromosomal integrations -
Uhlich, G.A., Chen, C. 2012. A cloning vector for creation of Escherichia coli lacZ translational fusions and generation of linear template for chromosomal integrations. Plasmid Journal. 67(3):259-263.
Sequence analysis of a group of low molecular-weight plasmids carrying multiple IS903 elements flanking a kanamycin resistance aph gene in Salmonella enterica serovars -
Chen, C., Strobaugh Jr, T.P., Lindsey, R.L., Frye, J.G., Uhlich, G.A. 2011. Sequence analysis of a group of low molecular-weight plasmids carrying multiple IS903 elements flanking a kanamycin resistance aph gene in Salmonella enterica serovars. Plasmid Journal. 65:246-252.
Comparative proteomics of E. coli O157:H7: two-dimensional gel electrophoresis vs. two-dimensional liquid chromatography separation -
Gunther, N.W., Pang, H., Nunez, A., Uhlich, G.A. 2010. Comparative proteomics of E. coli O157:H7: two-dimensional gel electrophoresis vs. two-dimensional liquid chromatography separation. Open Proteomics Journal. 3:26-34.
Esherichia coli serotype O157:H7 retention on solid surfaces and peroxide resistance is enhanced by dual-strain biofilm formation -
Uhlich, G.A., Rogers, D., Mosier, D.A. 2010. Esherichia coli serotype O157:H7 retention on solid surfaces and peroxide resistance is enhanced by dual-strain biofilm formation. Foodborne Pathogens and Disease. 7(8):935-943.
KatP contributes to OxyR-regulated hydrogen peroxide resistance in Escherichia coli serotype O157:H7 -
Uhlich, G.A. 2009. KatP contributes to OxyR-regulated hydrogen peroxide resistance in Escherichia coli serotype O157:H7. Microbiology. 155(11):3589-3598.
Molecular mechanisms involved in biofilm formation by food-associated bacteria -
Smith, J.L., Fratamico, P.M., Uhlich, G.A. 2009. Molecular mechanisms involved in biofilm formation by food-associated bacteria. Book Chapter. In: Fratamico, P.M., Annous, B.A., Gunther, N.W., editors. Biofilms in the Food and Beverage Industries. Oxford, UK: Woodhead Publishing, Ltd. p. 42-98.
The CsgA and Lpp proteins affect HEp-2 cell invasion, motility, and biofilm formation in a strain of Escherichia coli O157:H7 -
Uhlich, G.A., Gunther, N.W., Bayles, D.O., Mosier, D.A. 2009. The CsgA and Lpp proteins affect HEp-2 cell invasion, motility, and biofilm formation in a strain of Escherichia coli O157:H7. Infection and Immunity. 77(4):1543-1552.
Investigation of E. coli O157:H7 Mixed-Species Biofilm Formation -
Uhlich, G.A. 2008. Investigation of E. coli O157:H7 Mixed-Species Biofilm Formation. Meeting Abstract.
The CsgA and Lpp proteins affect motility and cell invasion in a strain of Escherichia coli O157:H7 -
Uhlich, G.A., Bayles, D.O., Mosier, D.A. The CsgA and Lpp proteins affect motility and cell invasion in a strain of Escherichia coli O157:H7. Meeting Abstract.
Characterization of Shiga toxin-producing Escherichia coli associated with two multi-state foodborne outbreaks in 2006 -
Uhlich, G.A., Sinclair, J.R., Warren, N.G., Chmielecki, W.A., Fratamico, P.M. 2008. Characterization of Shiga toxin-producing Escherichia coli associated with two multi-state foodborne outbreaks in 2006. Applied and Environmental Microbiology. 74:1268-1272.
Characterization of Shiga toxin-producing Escherichia coli isolated from different sources in 2006 -
Uhlich, G.A. 2007. Characterization of Shiga toxin-producing Escherichia coli isolated from different sources in 2006. Meeting Abstract.
Characterization of Shiga toxin-producing Escherichia coli isolated from different sources -
Uhlich, G.A. 2007. Characterization of Shiga toxin-producing Escherichia coli isolated from different sources. Meeting Abstract . Meeting Abstract.
The Lpp lipoprotein suppresses motility in a biofilm-forming strain of Escherichia coli O157:H7 -
Uhlich, G.A., Bayles, D.O. 2007. The Lpp lipoprotein suppresses motility in a biofilm-forming strain of Escherichia coli O157:H7. International Association of Food Protection. Abstract. P5-15.
ANALYSIS OF THE "RDAR" PHENOTYPE OF A STRAIN OF ESCHERICHIA COLI O157:H7 AND ITS ROLE IN BIOFILM FORMATION AND RESISTANCE TO ANTIBACTERIAL AGENTS -
Uhlich, G.A., Cooke, P.H., Solomon, E.B. 2006. Analysis of the "rdar" phenotype of a strain of escherichia coli o157:h7 and its role in biofilm formation and resistance to antibacterial agents. Applied and Environmental Microbiology. Vol. 72:2564-2572.
Analysis of biofilm formation by Escherichia coli serotype O157:H7 -
Uhlich, G.A. 2006. Analysis of biofilm formation by Escherichia coli serotype O157:H7. Meeting Abstract.
MUTATIONS IN CRP/FNR FAMILY OF REGULATORY GENES IN LISTERIA MONOCYTOGENES STRAIN F2365 DO NOT ALTER HEAT RESISTANCE AT 60 DEGREES C -
Bayles, D.O., Uhlich, G.A. 2006. Mutations in crp/fnr family of regulatory genes in listeria monocytogenes strain f2365 do not alter heat resistance at 60 degrees c. Journal of Food Protection. 69:2758-2760.
COMPARING TECHNIQUES FOR COMPARATIVE PROTEOMICS: TWO-DIMENSIONAL GEL ELECTROPHORESIS AND TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY SEPARATION -
Gunther, N.W., Hoan-Jen, P., Nunez, A., Uhlich, G.A. 2006. Comparing techniques for comparative proteomics: two-dimensional gel electrophoresis and two-dimensional liquid chromatography separation. Society of Industrial Microbiology. {Abstract}. P75.
EFFECT OF STORAGE TEMPERATURE ON THE VIABILITY OF LISTERIA MONOCYTOGENES IN QUESO BLANCO -
Uhlich, G.A., Luchansky, J.B., Tamplin, M.L., Molina-Corral, F.J., Anandan, S., Porto Fett, A.C. 2006. Effect of storage temperature on the viability of listeria monocytogenes in queso blanco. Journal of Food Safety. 26:202-214.
TRANSPOSON MUTAGENESIS REVEALS POTENTIAL SUPPRESSORS OF CURLI FIBERS IN EHEC STRAIN EDL933 -
Uhlich, G.A., Gunther, N.W. 2006. Transposon mutagenesis reveals potential suppressors of curli fibers in ehec strain edl933. {Abstract}. Society for Industrial Microbiology. P.82.
EFFECT OF STORAGE TEMPERATURE ON VIABILITY OF LISTERIA MONOCYTOGENES IN QUESO BLANCO -
Uhlich, G.A., Luchansky, J.B., Tamplin, M.L., Molina-Corral, F., Anandan, S., Porto Fett, A.C. 2006. Effect of storage temperature on viability of listeria monocytogenes in queso blanco. [Abstract] 12th Australian Food Microbiology Conference.
CHARACTERIZATION, TYPING, AND DETECTION OF PATHOGENIC ESCHERICHIA COLI -
Fratamico, P.M., Uhlich, G.A., Liu, Y. 2005. Characterization, typing, and detection of pathogenic escherichia coli. UJNR Food & Agricultural Panel Proceedings.
A PRFA TRANSPOSON MUTANT OF LISTERIA MONOCYTOGENES F2365, A SEROTYPE 4B STRAIN, IS ABLE TO SURVIVE IN THE GASTROINTESTINAL TRACT BUT NOT CAUSE SYSTEMIC INFECTION OF THE SPLEEN AND LIVER OF INTRAGASTRICALLY INOCULATED MICE -
Faith, N., Uhlich, G.A., Luchansky, J.B., Neudeck, B., Czuprynski, C. 2005. A prfa transposon mutant of listeria monocytogenes f2365, a serotype 4b strain, is able to survive in the gastrointestinal tract but not cause systemic infection of the spleen and liver of intragastrically inoculated mice. Infection and Immunity. 73:7517-7524.
Analyses of the putative Crp/Fnr family of transciptional regulators of a serotype 4B strain of Listeria monocytogens -
Uhlich, G.A., Wonderling, L., Luchansky, J.B. 2005. Transposon mutagenesis of a crp/fnr family of regulators from a serotype 4b strain of listeria monocytogenes. Food Microbiology. 23(2006):300-3006.
ANALYSES OF THE PUTATIVE CRP/FNR FAMILY OF TRANSCRIPTIONAL REGULATORS OF A SEROTYPE 4B STRAIN OF LISTERIA MONOCYTOGENES -
Uhlich, G.A., Wonderling, L., Luchansky, J.B. 2005. Analyses of the putative crp/fnr family of transcriptional regulators of a serotype 4b strain of listeria monocytogenes. Food Microbiology. Available: http://www.sciencedirect.com/science.
MUTATIONS IN CRP/FNR FAMILY OF REGULATORY GENES IN LISTERIA MONOCYTOGENES DO NOT ALTER THE HEAT RESISTANCE OF THE PATHOGEN -
Bayles, D.O., Uhlich, G.A. 2005. Mutations in crp/fnr family of regulatory genes in listeria monocytogenes do not alter the heat resistance of the pathogen. Meeting Abstract. Poster P1-11. pg. 69.
"OHMICS" AND CONTROL OF FOOD-BORNE PATHOGENS: EMPHASIS ON LISTERIA MONOCYTOGENES IN READY-TO-EAT MEATS -
Uhlich, G.A., Bayles, D.O., Gilbreth, S.E., Call, J.E., Luchansky, J.B. 2004. "ohmics" and control of food-borne pathogens: emphasis on listeria monocytogenes in ready-to-eat meats. United States Japan Natural Resources Protein Panel. Food and Agriculture Panel. p. 397-401
COMPARISON OF VIRULENCE IN MICE AND CACO-2 CELL INVASION BY MUTANTS OF THE PUTATIVE CRP/FNR FAMILY OF TRANSCRIPTIONAL REGULATORS OF A SEROTYPE 4B STRAIN OF LISTERIA MONOCYTOGENES -
Czuprynski, C., Uhlich, G.A., Wonderling, L.D., Faith, N., Neudeck, B., Loeb, J., Luchansky, J.B. 2004. Comparison of virulence in mice and caco-2 cell invasion by mutants of the putative crp/fnr family of transcriptional regulators of a serotype 4b strain of listeria monocytogenes. Meeting Abstract.
WHOLE GENOME COMPARISONS OF SEROTYPE 4B AND 1/2A STRAINS OF THE FOOD-BORNE PATHOGEN LISTERIA MONOCYTOGENES REVEAL NEW INSIGHTS INTO THE CORE GENOME COMPONENTS OF THIS SPECIES -
Nelson, K., Fouts, D., Mongodin, E., Fraser, C., Kathariou, S., Wonderling, L.D., Uhlich, G.A., Bayles, D.O., Luchansky, J.B. 2004. Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen listeria monocytogenes reveal new insights into the core genome components of this species. Proceedings Of The National Academy Of Sciences. 2004. Vol. 32. No. pg. 2386-2395.
FUNCTIONAL CHARACTERIZATION OF THE PUTATIVE CRP/FNR FAMILY OF TRANSCRIPTIONAL REGULATORS OF A LISTERIA MONOCYTOGENES SEROTYPE 4B STRAIN -
Uhlich, G.A., Wonderling, L., Faith, N., Neudeck, B., Loeb, J., Czuprynski, C., Luchansky, J.B. 2004. Functional characterization of the putative crp/fnr family of transcriptional regulators of a Listeria monocytogenes serotype 4b strain. Meeting Abstract. P174.
WHOLE GENOME SEQUENCING OF LISTERIA MONOCYTOGENES F2365, A SEROTYPE 4B STRAIN, AND COMPARISON TO OTHER L. MONOCYTOGENES STRAINS -
Fouts, D.E., Kolonay, J., Forberger, H.A., Khouri, H.M., Lindsay, J.A., Luchansky, J.B., Tran, B., Uhlich, G.A., Wonderling, L.D., Nelson, K.E. 2003. Whole genome sequencing of listeria monocytogenes f2365, a serotype 4b strain, and comparison to other l. monocytogenes strains. American Society of Microbiology. Abstract # R-058, p. 621.
IDENTIFICATION OF TWO PUTATIVE REGULATORY GENES REQUIRED FOR MOTILITY OF LISTERIA MONOCYTOGENES STRAIN F2365 -
Wonderling, L.D., Uhlich, G.A., Cooke, P.H., Luchansky, J.B. 2003. Identification of two putative regulatory genes required for motility of listeria monocytogenes strain f2365. American Society of Microbiology.Abstract # K126, p. 381.
ENVIRONMENTAL, HOST, AND BACTERIAL FACTORS THAT INFLUENCE THE PATHOGENESIS OF GASTROINTESTINAL LISTERIOSIS -
Czuprynski, C.J., Faith, N.G., Tamplin, M.L., Uhlich, G.A., Wonderling, L.D., Bayles, D.O., Luchansky, J.B. 2003. Environmental, host, and bacterial factors that influence the pathogenesis of gastrointestinal listeriosis. Meeting Abstract. Part 8,Section 15, No.3.
PHENOTYPIC ANALYSES OF THE PUTATIVE CRP/FNR FAMILY OF TRANSCRIPTIONAL REGULATORS IN A SEROTYPE 4B STRAIN OF LISTERIA MONOCYTOGENES -
Uhlich, G.A., Wonderling, L.D., Luchansky, J.B. 2003. Phenotypic analyses of the putative crp/fnr family of transcriptional regulators in a serotype 4b strain of listeria monocytogenes. American Society for Microbiology. Abstract #K-125, p. 381.
VARIATIONS IN THE CSGD PROMOTER OF ESCHERICHIA COLI O157:H7 ASSOCIATED WITHINCREASED VIRULENCE IN MICE AND INCREASED INVASION OF HEP-2 CELLS -
Infection and Immunity. 2002. v. 70. p. 395-399.
MUTATIONS IN THE CSGD PROMOTER ASSOCIATED WITH VARIATIONS IN CURLI EXPRESSION IN CERTAIN STRAINS OF ESCHERICHIA COLI O157:H7 -
PHASE VARIATION OF CURLI EXPRESSION IN STRAINS OF ESCHERICHIA COLI O157:H7 -
EFFECTS OF HAY- AND GRAIN-BASED DIETS ON FECAL SHEDDING OF NATURALLY- ACQUIRED ENTEROHEMORRHAGIC ESCHERICHIA COLI (EHEC) O157 IN BEEF FEEDLOT CATTLE -
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Last Modified: 5/2/2016
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