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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Molecular Characterization of Foodborne Pathogens Research » Research » Publications at this Location » Publication #345589

Research Project: Shiga Toxin-Producing Escherichia coli in Biofilms and within Microbial Communities in Food

Location: Molecular Characterization of Foodborne Pathogens Research

Title: Whole-genome sequence of Escherichia coli serotype O157:H7 strain B6914-ARS

Author
item Uhlich, Gaylen
item Reichenberger, Erin
item Cottrell, Bryan
item Fratamico, Pina
item Andreozzi, Elisa - Collaborator

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/28/2017
Publication Date: 11/1/2017
Citation: Uhlich, G.A., Reichenberger, E.R., Cottrell, B.J., Fratamico, P.M., Andreozzi, E. 2017. Whole-genome sequence of Escherichia coli serotype O157:H7 strain B6914-ARS. Genome Announcements. https://doi.org/10.1128/genomeA.01191-17.

Interpretive Summary: Escherichia coli serotype O157:H7 strain B6-914 is a human fecal isolate that was submitted to the Centers for Disease Control and Prevention and later deposited with the American Type Culture Collection (ATCC) as strain ATCC 43888. It is less harmful to laboratory personnel since it is an E. coli O157:H7 strain that lacks the genes that encode for Shiga toxins, resulting in its widespread use in applied microbiology research. A B6-914 isolate in one USDA culture collection (designated B6-914ARS) has been shown to have unique properties with regard to the ability to form biofilms (bacteria within a complex structure adhering to surfaces) and persist on food processing surfaces that likely stem from variations in genomic regions that carry bacteriophage (viruses that infect bacteria) insertions. Therefore, the entire genome sequence (genetic material of the bacterium) of strain B6-914ARS was determined and analyzed. The information is valuable for comparative studies with other E. coli O157:H7 genome sequences and for determining how O157:H7 strains form biofilms and persist in food environments.

Technical Abstract: Escherichia coli serotype O157:H7 strain B6914-MS1 is a Shiga toxin-deficient human fecal isolate obtained by the Centers for Disease Control and Prevention that has been used extensively in applied research studies. Here we report the genome sequence of strain B6914-ARS, a B6914-MS1 clone that has unique biofilm properties.