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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 8/23/2006 Publication Date: 8/28/2006 Citation: Uhlich, G.A. 2006. Analysis of biofilm formation by Escherichia coli serotype O157:H7. Meeting Abstract. Interpretive Summary: Technical Abstract: We identified Congo red-binding (curli-producing) and -nonbinding phase variants of Escherichia coli O157:H7 strain ATCC 43895. The Congo red-binding variant, strain 43895OR, produced a red, dry and rough (rdar) phenotype on low-salt agar containing Congo red, and generated abundant biofilms on glass, polystyrene, and stainless steel. In contrast, variant 43895OW produced a smooth and white (saw) colony morphology and generated 5-8 fold less biofilm on the same surfaces. To better understand the genetic mechanisms involved in biofilm formation, we compared both variants using a variety of proteomic and genomic techniques. To date we have identified more than 20 proteins that are up-regulated in each variant during growth on low-salt agar. In addition, gene profiling experiments using reverse-transcriptase real-time PCR indicate that the regulation of biofilm formation in strain 43895OR is distinct from that previously described for other strain of E. coli. |
