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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Molecular Characterization of Foodborne Pathogens Research » Research » Publications at this Location » Publication #378036

Research Project: Shiga Toxin-Producing Escherichia coli in Biofilms and within Microbial Communities in Food

Location: Molecular Characterization of Foodborne Pathogens Research

Title: Escherichia coli serotype O157:H7 PA20R2R complete genome sequence

item Uhlich, Gaylen
item Paoli, George
item Kanrar, Siddhartha

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/23/2020
Publication Date: 12/10/2020
Citation: Uhlich, G.A., Paoli, G., Kanrar, S. 2020. Escherichia coli serotype O157:H7 PA20R2R complete genome sequence. Microbiology Resource Announcements. 9(50): e01143-20.

Interpretive Summary: O157:H7 is the most common Escherichia coli serotype associated with severe bloody diarrheal disease in humans. When O157:H7 strains infect humans (clinical strains), they modify their DNA to reduce surface properties in order to avoid detection and clearance from the body. However, these changes reduce their ability to survive outside the body. Strain 20R2R is an O157:H7 strain derived from a clinical strain that made additional changes to its genome that restored some properties favoring survival after leaving its human host. We report here the entire DNA sequence of strain 20R2R to aid investigations of the DNA changes and bacterial properties that allow O157:H7 to survive when moving between humans and the environment.

Technical Abstract: Escherichia coli serotype O157:H7 strain 20R2R is a derivative of clinical isolate PA20. Prophage excision from the coding region of a PA20 transcription factor restored RpoS-dependent biofilm formation in 20R2R, providing a model for O157:H7 stress adaptation when transitioning between clinical and environmental settings. We report here the complete 20R2R genome sequences.