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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Characterization and Interventions for Foodborne Pathogens » Research » Publications at this Location » Publication #407007

Research Project: Molecular Analysis of Foodborne Pathogen Responses to Stressors

Location: Characterization and Interventions for Foodborne Pathogens

Title: Whole genome sequencing and characterization of Escherichia coli human isolate DP033

item Liu, Yanhong
item Kanrar, Siddhartha
item ELDER, JACOB - Collaborator
item Ream, Amy
item Huang, Lihan
item Gehring, Andrew
item Uhlich, Gaylen
item CLABOTS, CONNIE - University Of Minnesota
item JOHNSON, JAMES - University Of Minnesota

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/19/2023
Publication Date: 12/15/2023
Citation: Liu, Y., Kanrar, S., Elder, J., Ream, A.R., Huang, L., Gehring, A.G., Uhlich, G.A., Clabots, C., Johnson, J.R. 2023. Whole genome sequencing and characterization of Escherichia coli human isolate DP033. Microbiology Resource Announcements. Available online: January 2024 13 Issue 1.

Interpretive Summary: Escherichia coli strains found in food such as chicken meat can potentially cause extra-intestinal infections in humans. These strains are referred to as extra-intestinal pathogenic E. coli (ExPEC). Strain DP033 was isolated from a human and was shown to contain virulence genes (genes involved in causing disease) that are found in typical ExPEC strains. This strain was also shown to be virulent using a cell adhesion assay. Because this presumptive ExPEC strain was a clinical isolate, it was characterized to determine its virulence potential using whole genome sequencing. Genomic analysis was used to determine the characteristics of this strain, including possession of a number of virulence genes and antibiotic resistance genes. The whole genome data are useful for understanding ExPEC pathogenesis if ExPEC strains from food can cause illness in humans.

Technical Abstract: The whole genome sequence of Escherichia coli strain DP033 is reported here. DP033 was isolated from a human rectal specimen in Tilburg, Netherlands. An adhesion assay with Caco-2 cells showed that DP033 had a 6.3-fold higher adhesion capacity compared to that of an E. coli O157:H7 strain. In addition, whole genome sequencing data showed that DP033 possessed 36 virulence-related genes and is a potential extraintestinal pathogenic E. coli (ExPEC) strain.