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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Molecular Characterization of Foodborne Pathogens Research » Research » Publications at this Location » Publication #366144

Research Project: Shiga Toxin-Producing Escherichia coli in Biofilms and within Microbial Communities in Food

Location: Molecular Characterization of Foodborne Pathogens Research

Title: Whole-genome sequence of Escherichia coli serotype O157:H7 strain ATCC 43888

item Uhlich, Gaylen
item Paoli, George
item ZHANG, XINMIN - Bioinforx Inc
item ANDREOZZI, ELISA - Collaborator

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/30/2019
Publication Date: 10/19/2019
Citation: Uhlich, G.A., Paoli, G., Zhang, X., Andreozzi, E. 2019. Whole-genome sequence of Escherichia coli serotype O157:H7 strain ATCC 43888. Genome Announcements. Available: Microbiol Resources Announcement 8:e00906-19.

Interpretive Summary: Pathogenic Shiga toxin-producing Escherichia coli (STEC) O157:H7 was first reported in the early 1980’s. Since then many types of STEC have been reported, but STEC O157:H7 remains the leading cause of foodborne illnesses, deaths and hospitalizations. For safety reasons, studies of STEC O157:H7 are often done using strains deficient in different virulence factors. Strain B6914-MS1, is a toxin-deficient human fecal isolate that has been used extensively for research because it was made widely available through a large curated bacterial culture collection, The American Type Culture Collection (ATCC), as strain ATCC 43888. Strains of STEC O157:H7 are genetically diverse, showing large genomic differences between strains; thus, the genome sequence from a single O157:H7 strain is not be representative. Here, we report the complete genome sequence of STEC O157:H7 strain ATCC 43888 which was deposited in the GenBank public genome database. The genome sequence will be helpful to us and other researchers who use strain ATCC 43888 as a model for the behavior of E. coli O157:H7 in foods.

Technical Abstract: Escherichia coli serotype O157:H7 strain ATCC 43888 is a Shiga toxin-deficient human fecal isolate. Due to its reduced toxicity and availability from a curated culture collection, the strain has been used extensively in applied research studies. Here we report the Illumina-corrected PacBio whole-genome sequence of E. coli O157:H7 strain ATCC 43888.