Location: Grain Quality and Structure ResearchTitle: Optimization of ethanol-based extraction conditions of sorghum bran bioactive compounds with downstream anticancer properties
|LEE, SEONG-HO - University Of Maryland|
|PERUMAL, RAMASAMY - Kansas State University|
Submitted to: Heliyon
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/24/2019
Publication Date: 5/7/2019
Citation: Cox, S.R., Noronha, L.E., Herald, T.J., Bean, S.R., Lee, S., Perumal, R., Smolensky, D. 2019. Optimization of ethanol-based extraction conditions of sorghum bran bioactive compounds with downstream anticancer properties. Heliyon. https://doi.org/10.1016/j.heliyon.2019.e01589.
Interpretive Summary: Some sorghum bioactive compounds with anticancer activity have been identified. Although, safe solvents to improve extraction of these compounds for screening need further exploration. The aim of this study was to optimize the extraction of health benefiting compounds from sorghum grain using food-grade solvents. The extracts were screened for anticancer properties in two cancer cell lines, using a cell viability assay. The sorghum grain with highest amount of health promoting compounds was more effective at inhibiting cell viability than the other samples. The results demonstrate an efficient method was developed for extracting sorghum bioactive compounds for future anticancer research.
Technical Abstract: Certain foods such as turmeric and green tea have been extensively for anticancer properties, while high polyphenol sorghum has not received the same attention. Some sorghum bioactive compounds with anticancer activity have been identified, indicating the further need for research and screening methods of high polyphenol sorghum genotypes. This study was aimed at improving the extraction of sorghum bioactive compounds by using ethanol and citric acid as food-grade solvents. We used bran from three sorghum genotypes [(PI570481 (HP), Sumac (CS) and black RTx430 (CB)] and green tea (GT) as a control. The extraction methods were screened for anticancer properties in HepG2 and HCT-15 cancer cell lines, using a cell viability assay. Extraction conditions were optimized for anticancer compounds from a high-phenolic sorghum HP, CS, and GT. HP was more effective at inhibiting cell viability than CB, CS, and GT. The results demonstrate an efficient method for extracting sorghum bioactive compounds for future anticancer research.