|GAUDINIER, ALLISON - University Of California|
|ZHANG, LIFANG - Cold Spring Harbor Laboratory|
|REECE-HOYES, JOHN - University Of Massachusetts|
|TAYLOR-TEEPLES, MALLORIE - University Of California|
|PU, LI - University Of California|
|LIU, ZHIJIE - Cold Spring Harbor Laboratory|
|BRETON, GHISLAIN - University Of California|
|PRUNEDA-PAZ, JOSE - University Of California|
|KIM, DAHAE - University Of California|
|KAY, STEVE - University Of California|
|WALHOUT, ALBERTHA - University Of Massachusetts|
|BRADY, SIOBHAM - University Of California|
Submitted to: Nature Methods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/30/2011
Publication Date: 10/30/2011
Citation: Gaudinier, A., Zhang, L., Reece-Hoyes, J.S., Taylor-Teeples, M., Pu, L., Liu, Z., Breton, G., Pruneda-Paz, J.L., Kim, D., Kay, S.A., Walhout, A.J., Ware, D., Brady, S.M. 2011. Enhanced Y1H Assays for Arabidopis. Nature Methods. 8:1053-1055.
Interpretive Summary: An Arabidopsis full-length enhanced yeast one hybrid (eY1H) transcription factor (TF) resource comprised of 645 or 91% of TFs expressed in the root stele and 75% of root-expressed TFs is presented. We demonstrate that this resource and the eY1H assay enable rapid, efficient and systematic mapping of plant TF-promoter interactions using thirteen stele-expressed TF promoters. 158 interactions were identified, many of which occur physically or are regulatory in planta.
Technical Abstract: Transcription regulation plays a key role in development and response to environment. To understand this mechanism, we need to know which transcription factor (TFs) would bind to which promoter, thus regulate their target gene expression. Yeast one-hybrid (Y1H) technique can be used to map this kind of interaction. This paper described the improvement of this method as enhanced Y1H (eY1H), using robotic mating method to replace laborious transformation method, thus more efficient and reduce the cost. Most importantly, this paper provided a more complete TF resource (including 69 de novo cloning of TFs) that represent 91% of TFs that expressed in root stele and 73% of TFs expressed in root. It would be great resource to the plant community.