Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 12/30/2009
Publication Date: 5/8/2009
Citation: Susta, L., Harrison, L., Zhang, J., Afonso, C.L., Miller, P.J., Brown, C.C. 2009. Presence of apoptosis, as determined by immunohistochemistry, in lymphoid tissues of chickens infected with strains of Newcastle disease virus of varying virulence [abstract]. American Association of Avian Pathologists/American Veterinary Medical Association Annual Meeting, July 11-15, 2009, Seattle, Washington. p. 16.
Technical Abstract: Virus-induced apoptosis is an anti-viral mechanism of host defense utilized by eukaryotic cells to minimize viral replication and to reduce damage caused by infection while clearing the invading pathogen. Thus, we have analyzed the expression of caspases, key enzymes in the induction of apoptosis. Formalin-fixed, paraffin embedded sections from tissues of chickens infected with 8 Newcastle disease virus (NDV) strains of different virulence were evaluated via immunohistochemistry (IHC) to detect the active isoforms of various caspases in the main lymphoid organs. The number of positive cells was numerically assessed and the differences in expression were statistically evaluated.