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Title: Transcriptional analysis of the innate immune response using the avian innate immunity microarray

Author
item CHUNG, IDA H. - UNIV OF DELAWARE
item KEELER, CALVIN - UNIV OF DELAWARE
item Kapczynski, Darrell
item DOUGHTERY, LORNA - UNIV OF DELAWARE
item MAUGHAN, MICHELE - UNIV OF DELAWARE

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/30/2008
Publication Date: 6/1/2008
Citation: Chung, I.T., Keeler, C.L., Kapczynski, D.R., Doughtery, L., Maughan, M.N. 2008. Transcriptional analysis of the innate immune response using the avian innate immunity microarray [abstract]. Immunobiology and Pathogenesis of Influenza Infection meeting, June 1-3, 2008, Atlanta, Georgia. CDROM.

Interpretive Summary:

Technical Abstract: The avian innate immunity microarray (AIIM) is a genomics tool designed to study the transcriptional activity of the avian immune response (Cytogenet. Genome Res. 117:139-145, 2007). It is an avian cDNA microarray representing 4,959 avian genes spotted in triplicate. The AIIM contains 25 avian interleukin, chemokine, and cytokine elements as well as elements involved in several innate immune pathways, apoptosis, and antigen presentation. In this study, six four-week-old white leghorn chickens received either A/Egret/HK/757.2/02 (HPAI H5N1) or mock inoculation (PBS). RNA was extracted from spleen tissue after 24 hours post inoculation, and analyzed using the AIIM. Analysis of the results generated indicated that 120 elements were induced 2-fold or greater while 318 elements were repressed at least 2-fold (p<0.05) following AI-infection compared to mock. Indicative of an avian immune response to viral infection, several genes were highly induced including IFIT (35-fold), a key factor in the interferon response, MIP-1beta (5-fold), an important chemokine involved in the inflammatory response, and TLR3 (4-fold), a receptor known to respond to influenza infection.