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ARS Home » Research » Publications at this Location » Publication #204747

Title: Real time RT-PCR for the H5 HA subtype

item Spackman, Erica
item Suarez, David

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 7/12/2007
Publication Date: 3/1/2008
Citation: Spackman, E., Suarez, D.L. 2008. Detection and identification of the H5 hemagglutinin subtype by real-time RT-PCR. In: Spackman, E., editor. Avian Influenza Virus Methods. Totowa, NJ: Humana press. p. 27-34.

Interpretive Summary:

Technical Abstract: Real-time RT-PCR (RRT-PCR) for type A influenza detection is widely used with subsequent tests for subtype identification. Due to the importance of the H5 HA subtype, rapid and sensitive detection of the H5 HA subtype is particularly useful due to the importance of the recent Asian H5N1 HPAI viruses. The procedure for real-time RT-PCR identification of the H5 HA subtype of any lineage is described here.