Submitted to: Analytical and Bioanalytical Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/9/2007
Publication Date: 8/21/2007
Citation: Xu, J., Suarez, D.L., Gottfried, D.S. 2007. Detection of avian influenza virus using an interferometric biosensor. Analytical and Bioanalytical Chemistry. 389:1193-1199. Interpretive Summary: Avian influenza virus can cause serious disease outbreaks in poultry. For control during an outbreak is to have a rapid detection when the birds are infected so the virus does not have a chance to spread. The paper describes the development of a new type of diagnostic that can potentially provide rapid and sensitive diagnosis of avian influenza. Additionally, this testing can potentially be done in the field because the equipment is portable, reasonable cost, and the sample requires little processing. This test has the potential to improve our ability to diagnosis avian influenza during outbreaks.
Technical Abstract: An optical interferometric waveguide immunoassay for direct and label-less detection of avian influenza virus is described. The assay response is based on index of refraction changes that occur upon binding of virus particles to antigen (hemagglutinin) specific antibodies on the waveguide surface. Three virus subtypes (two H7 and one H8) in buffer solution were tested using both monoclonal and polyclonal capture antibodies. The real-time response of the antigen-antibody interaction was measured and shown to be concentration dependent, with detection limits as low as .0005 HA units/ml. A simple sandwich assay was shown to further increase the biosensor response.