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Title: REAL TIME RT-PCR DEVELOPED FOR A PHYLOGENETICALLY DIVERGENT GROUP OF NEWCASTLE VIRUSES NOT DETECTED BY CURRENT TESTS

Author
item Kim, L
item Afonso, Claudio
item Suarez, David
item King, Daniel

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2006
Publication Date: 6/26/2006
Citation: Kim, L.M., Afonso, C.L., Suarez, D.L., King, D.J. 2006. Real time RT-PCR developed for a phylogenetically divergent group of Newcastle viruses not detected by current tests [abstract]. American Association of Avian Pathologists. CD p. 156.

Interpretive Summary:

Technical Abstract: Newcastle disease virus, also known as avian paramyxovirus-1 (APMV-1), is an important poultry pathogen worldwide. Sensitive rapid diagnostic tests exist to detect APMV-1; however, due to the heterogeneous genetic nature of this virus, a unique and phylogenetically separated group of low-virulence viruses may be missed with current tests. These viruses are commonly found in waterfowl and shorebirds, but are also known to infect domestic poultry with at least some being virulent. This study outlines the development of a primer and probe set that detects members of this divergent group. Additionally, this set is compatible with the currently validated matrix real-time RT-PCR as a multiplex test.