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Title: ELISA TEST FOR THE DIFFERENTIATION OF INFECTED AND VACCINATED ANIMALS (DIVA) USING A NATURAL TRUNCATED NS1 PROTEIN OF AVIAN INFLUENZA VIRUS

Author
item Avellaneda, Gloria
item Lee, Chang
item Suarez, David

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 1/8/2006
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The control of Avian Influenza Virus has relied mainly on quarantine and elimination of infected flocks. This strategy allows countries to quickly eliminate disease and return to normal trade. Vaccination has not been more commonly used for control because current killed vaccines interfere with routine serologic surveillance and therefore, with the ability to export poultry. A DIVA (Differentiate Infected from Vaccinated Animals) approach with natural variant of the NS1 protein has previously been reported, but because of how poultry vaccines are made, this NS1 approach had limitations. An alternative DIVA strategy using a virus with a naturally truncated NS1 gene has been developed. In this strategy, the deleted fragment of the truncated NS1 protein used in an ELISA test provides clearer differentiation of vaccinated and infected birds, when the tested flocks have been vaccinated with a virus containing the natural truncated gene. Preliminary results will be presented and their impact on the actual Avian Influenza situation will be discussed.