Submitted to: Veterinary Pathology
Publication Type: Abstract Only
Publication Acceptance Date: 7/1/2004
Publication Date: 11/10/2004
Citation: Piacenti, A.M., King, D.J., Seal, B.S., Brown, C.C. 2004. Pathogenesis of newcastle disease in turkeys experimentally infected with different isolates [abstract]. Veterinary Pathology. 41(5):573.
Technical Abstract: Newcastle disease is a limiting disease of poultry worldwide and the cause of significant economic impact when outbreaks occur. The etiology, Newcastle disease virus (NDV), infects a wide range of avian species and has been well studied in chickens. However, the pathogenesis of NDV in turkeys is poorly understood. Control of an outbreak in a turkey-producing area will require more thorough appreciation of the action of the virus in this species. In this study, groups of four week old commercial turkeys were inoculated intraconjunctivally with 5 viral isolates (LaSota, Roakin, Turkey North Dakota, Iowa 1519 and CA 1083) representing all pathotypes of NDV. Clinical signs were monitored daily and 2 birds from each group were euthanatized, with collection of tissues into formalin on days 2, 5, 10 and 14 postinoculation. The tissues were examined by histopathology, immunohistochemistry for the presence of NDV nucleoprotein, and in situ hybridization for the presence of viral mRNA. No abnormal clinical signs were noted in birds infected with lentogenic strains; varying degrees of depression and nervous signs were observed in some birds of the mesogenic and velogenic groups.