Submitted to: Anaerobe
Publication Type: Peer reviewed journal
Publication Acceptance Date: 1/3/2005
Publication Date: 3/29/2005
Citation: Russell, J.B. 2005. Enrichment of fusobacteria from the rumen that can utilize lysine as an energy source for growth. Anaerobe 11:177-184. Interpretive Summary: Ruminal lysine degradation is a wasteful process that deprives the animal of an essential amino acid, but it was not clear which of the bacteria were responsible for this degradation. Lysine degrading bacteria were enriched from the rumen and isolated. 16S rDNA sequencing indicated that they were strains of Fusobacterium necrophorum. Growing cultures had varying rates of lysine degradation, but lysine transport could be inhibited by either acidic pH or the feed additive monensin. Research on ruminal amino acid deamination has the potential to decrease the cost of cattle production.
Technical Abstract: Ruminal lysine degradation is a wasteful process that deprives the animal of an essential amino acid. Mixed ruminal bacteria did not deaminate lysine (50 mM) at a rapid rate, but lysine degrading bacteria could be enriched if Trypticase (5 mg ml-1) was also added. Lysine degrading isolates produced acetate, butyrate and ammonia, were non-motile, stained gram-negative and could also utilize lactate, glucose, maltose or galactose as an energy source for growth. Lactate was converted to acetate and propionate, and 16S rDNA indicated that their closest relatives were Fusobacterium necrophorum. Growing cultures produced ammonia at rates as high as 2400 nmol mg protein ml-1min-1. Washed cell suspensions took up 14C lysine (3 M) at an initial rate of 6 nmol mg protein-1 min-1, and glucose addition did not affect the transport. Cells washed aerobically had the same transport rate as those handled anaerobically, but only if the transport buffer contained sodium. The affinity constant for sodium was 8 mM, and sodium could not be replaced by lithium. Cells treated with the sodium/proton antiporter, monensin (5 M), did not take up lysine, but a protonophore that inhibited growth (tetrachlorosalicylanilide, 10 M) had no effect. An artificial membrane potential created by potassium diffusion did not increase the rate of lysine transport, and an Eadie-Scatchard plot indicated the transport rate was directly proportional to the lysine concentration. Decreasing the pH from 6.7 to 5.5 caused an 85% decrease in the rate of lysine transport. The addition of F. necrophorum JB2 (130 g protein ml-1) to mixed ruminal bacteria increased lysine degradation 10-fold, but only if the pH was 6.7 and monensin was not present. Further work will be needed to see if dietary lysine enriches fusobacteria in vivo.