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Title: NDV REAL-TIME RT-PCR TEST DEVELOPMENT, VALIDATION AND USE AS A NATIONAL TEST AT NVSL

Author
item Spackman, Erica
item Wise, Mark
item Suarez, David
item SENNE, D - NVSL, AMES, IA
item PEDERSEN, J - NVSL, AMES, IA
item King, Daniel
item Swayne, David
item SCHMITT, B - NVSL, AMES, IA
item Seal, Bruce

Submitted to: United States Animal Health Association Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/3/2003
Publication Date: 10/16/2003
Citation: Spackman, E., Wise, M., Suarez, D.L., Senne, D.A., Pedersen, J.C., King, D.J., Swayne, D.E., Schmitt, B.J., Seal, B.S. NDV Real-Time RT-PCR Test Development, Validation and Use As A National Test at NVSL. 2003. United States Animal Health Association Proceedings.

Interpretive Summary:

Technical Abstract: A real-time RT-PCR test for the detection of Avian Paramyxovirus type 1 (APMV-1) or Newcastle disease virus (NDV), has been developed, validated and implemented. Real-time RT-PCR, which involves the detection of a PCR product in real-time, is a rapid diagnostic method which offers a variety of advantages over virus isolation in embryonating chicken eggs, which is the current 'gold standard' for NDV detection. Speed is probably the most important advantage of the RRT-PCR technology as results may be obtained within three hours of sample collection, as opposed to virus isolation which takes a minimum of five days. The validation and application of this test will be discussed.