THE COMPLETE GENOME SEQUENCE OF THE ARABIDOPSIS AND TOMATO PATHOGEN PSEUDOMONAS SYRINGAE PV. TOMATODC3000

Authors: BUELL, C - INST GENOMIC RESEARCH; JOARDAR, V - INST GENOMIC RESEARCH; D'ASCENZO, M - BOYCE THOMPSON INST; DEGN, W - CORNELL UNIVERSITY; RAMOS, A - CORNELL UNIVERSITY; ALFANO, J - UNIVERSITY OF NEBRASKA; Cartinhour, Samuel; DELANEY, T - CORNELL UNIVERSITY; LAZAROWITZ, S - CORNELL UNIVERSITY; MARTIN, G - BOYCE THOMPSON INST; Schneider, David; COLLMER, A - CORNELL UNIVERSITY

Submitted to: Proceedings of the National Academy of Sciences (PNAS)
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/2/2003
Publication Date: 9/2/2003
Citation: BUELL, C.R., JOARDAR, V., D'ASCENZO, M., DEGN, W.L., RAMOS, A.R., ALFANO, J.R., CARTINHOUR, S.W., DELANEY, T.P., LAZAROWITZ, S.G., MARTIN, G.B., SCHNEIDER, D.J., COLLMER, A. THE COMPLETE GENOME SEQUENCE OF THE ARABIDOPSIS AND TOMATO PATHOGEN PSEUDOMONAS SYRINGAE PV. TOMATODC3000. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES. 2003. v. 100(18). p. 10181-10186.

Interpretive Summary: The complete genome sequence of the bacterial plant pathogen Pseudomonas syringae has been determined. The organism contains approximately 5,800 genes, out of which nearly 300 are probably directly associated with plant disease. The P. syringae genome also contains a large number of genes that help the organism survive in diverse environments, such as genes involved in the uptake of nutrients. Analysis shows that the genome of P. syringae is highly similar to two other fully sequenced members of the Pseudomonas group, P. putida and P. aeruginosa. However, P. syringae also contains over 1000 unique genes, many of which have no known function.

Technical Abstract: We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome (6.5 megabases) contains a circular chromosome and two plasmids, which collectively encode 5,763 ORFs. We identified 298 established and putative virulence genes, including several clusters of genes encoding 31 confirmed and 19 predicted type III secretion system effector proteins. Many of the virulence genes were members of paralogous families and also were proximal to mobile elements, which collectively comprise 7% of the DC3000 genome. The bacterium possesses a large repertoire of transporters for the acquisition of nutrients, particularly sugars, as well as genes implicated in attachment to plant surfaces. Over 12% of the genes are dedicated to regulation, which may reflect the need for rapid adaptation to the diverse environments encountered during epiphytic growth and pathogenesis. Comparative analyses confirmed a high degree of similarity with two sequenced pseudomonads, Pseudomonas putida and Pseudomonas aeruginosa, yet revealed 1,159 genes unique to DC3000, of which 811 lack a known function.