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item Kapczynski, Darrell
item Tumpey, Terrence

Submitted to: Western Poultry Disease Conference
Publication Type: Proceedings
Publication Acceptance Date: 3/8/2003
Publication Date: 3/8/2003
Citation: Kapczynski, D.R., Tumpey, T. 2003. Vaccination Efforts With Virosomes Produced From Newcastle Disease Virus. Western Poultry Disease Conference Proceedings, p.33, 2003.

Interpretive Summary: Not required.

Technical Abstract: Outbreaks of highly virulent Newcastle disease virus (NDV) occur with regular frequency and are a major concern to the poultry industry internationally. Economic losses caused by low virulent (lentogenic) strains continue to result from decreased egg production in layers and airsacculitis in broilers. In an effort to protect chickens against Newcastle disease (ND), a non-replicating virosome-vaccine was produced by solubilization of Newcastle disease virus (NDV) with Triton X-100 followed by detergent removal with SM2 Bio-Beads. Biochemical analysis indicated that the NDV virosomes had similar characteristics as the parent virus and contained both the fusion (F) and hemagglutinin-neuraminidase (HN) proteins. To target the respiratory tract, SPF chickens were immunized intranasally and intratracheally with the NDV virosome vaccine. This was compared to a standard NDV (LaSota) live-virus vaccine for commercial poultry. Seroconversion (> 4-fold increase in hemagglutination inhibition (HI) antibody titers) was achieved in all vaccinated birds utilizing the virosome vaccine. Upon lethal challenge with a velogenic NDV strain (Texas GB), all birds receiving either vaccination method were protected against death. Antibody levels against NDV, as determined by ELISA and HI titer, were comparable when utilizing either vaccine and increased following virus challenge. These results demonstrate the potential of virosomes as an effective tool for ND vaccination.