Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/15/2003
Publication Date: 4/15/2003
Citation: Zaripheh, S., Cooke, P., Novotny Dura, J., Erdman, J.W. 2003. The time course distribution of a single dose of 14c-lycopene in the f344 rat. Meeting Abstract.
Interpretive Summary: none
Technical Abstract: The objective of this study was to evaluate the time course distribution of a single dose of 14C-lycopene in F344 rats prefed lycopene (0.25g lyc/kg diet) for 30 days. Particular attention was given to the appearance of 14C labeled oxidation products in liver, serum, seminal vesicles and the prostate. Group of rats (n=8) were euthanized at 0, 3, 6, 24, 72 and 168 hours following the dose. While the liver contained the majority of 14C at all time points, it maintained about 80% all-trans/cis isomers of 14C. All-trans/cis 14C in serum decreased over time and oxidative products increased from 3-24 hours and then decreased. At 3 hours the 14C in seminal vesicles was primarily in the all-trans/5-cis form (70%) but by 168 hrs, 52% of 14C was found as oxidized products. Major cis isomers in the seminal vesicles appeared to increase, particularly after 72 hours. Despite the presence of unlabeled lycopene in the prostate, the primary form of 14C was oxidative products even at 3 hours. The percentage and amount of oxidation products of 14C in prostate increased with time. With the prostate, the oxidative products were greatest at all time points (77%-90% respectively). Compartmental analysis of serum and tissue 14C response modeling software is underway. The data suggest that in lycopene prefed rats, pathways are induced to metabolize lycopene to more polar compounds, which could lead to their elimination from the body.