An official website of the United States government
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
IgE binding epitope mapping with TL1A tagged peptides Linear IgE epitopes play essential roles in peanut and tree nut allergies. Overlapping peptides spanning the entire length of the protein sequences of food allergens were used in dot blot and microarray experiments, and the dominant linear epitopes of peanut allergens Ara h 1-3 were identified. Howe...
Food allergens of plant origin The prevalence of food allergies caused by adverse immune responses to allergenic food proteins has increased significantly in the past two to three decades. Food allergens belong to a tiny subset of about eighteen thousand protein families. While some of these families are well known to contain foo...
Screening of nanobody specific for peanut major allergen Ara h 3 by phage display Peanut allergy is a major health problem worldwide. Detection of food allergens is a critical aspect of food safety. The VHH domain of single chain antibody from camelids, also known as nanobody (Nb), showed its advantages in the development of biosensors because of its high stability, small molecul...
Almond (prunus dulcis) allergen pru du 8, the first member of a new family of food allergens families. Four almond allergens are designated by WHO/IUIS. Another almond allergen with two known short peptide sequences was reported as the 2S albumin but later suspected as vicilin. Whether this almond allergen in question was the albumin, vicilin, or another uncovered protein remains to be inve...
Structure, Function and Epitope Mapping the Peanut Panallergen Ara h 8 The incidence of peanut allergy continues to rise in the US and Europe. Whereas exposure to the major allergens Ara h 1, 2, 3, and 6 can cause fatal anaphylaxis, exposure to the minor allergens usually does not. Ara h 8 is a minor allergen. Importantly, it is the minor food allergens that are though...
A cashew specific monoclonal antibody recognizing the small subunit of Ana o 3 Food allergies represent a significant medical burden and preventing accidental exposure to food allergens requires constant attention. Reactions to cashew nuts can often be severe, and the small 2S albumin, Ana o 3, is an immuno-dominant cashew allergen. Ana o 3, like other 2S albumins, is compos...
Crystal structure of cocosin, a potential food allergen from coconut (Cocos nucifera) (abstract) RATIONALE: Coconut allergy cases have been reported, but only one coconut allergen has been identified. The 11S seed storage proteins belong to one of a few protein families that contain known food allergens in many food of plant sources. Cocosin, the 11S protein from cocosin remains to be character...
Recent development in recombinant food allergen production (abstract) Whether for understanding the properties of food allergens or for manufacturing vaccines for allergen-specific immunotherapy, well characterized pure allergens are required. This often necessitate the use of recombinant technology in obtaining food allergens due to the very low amounts of their natu...
Characterization of maize chitinase-A, a tough allergenic molecule Food allergies are recognized as an increasing health concern. Proteins commonly identified as food allergens tend to have one of about 30 different biochemical activities. This leads to the assumption that food allergens must have specific structural features which causes their allergenicity. But t...
Identification of a thermal processing-induced modification site on the Ana o 3 cashew allergen Cashew nuts are a common cause of food allergy and reactions to cashew nuts can be severe. Thermal processing can alter the properties of food allergens including their structure, solubility, and cause non-enzymatic reactions between reactive sugar carbonyl groups and amino groups within proteins. ...
New tree nut allergens The 7S vicilin and 11S legumin seed storage globulins belong to the cupin protein superfamily and are major food allergens in many of the “big eight” food allergen groups. Korean pine vicilin and pecan vicilin are thus predicted to be food allergens. Recombinant vicilins were expressed in E. coli an...
New structural information on food allergens (abstract) A small number of protein families are responsible for food allergies suffered by the majority of allergy patients. What properties of these proteins make them allergens is not clear at present. Reliable methods for allergen prediction and mitigation are lacking. Most the immediate type of food alle...
Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4 Biochemical characterizations of food allergens are required for understanding the allergenicity of food allergens. Such studies require a relatively large amount of highly purified allergens. Profilins from numerous species are known to be allergens, including food allergens, such as almond (Prunus...