Location: Healthy Processed Foods ResearchTitle: Recent development in recombinant food allergen production (abstract) Author
|Fan, Yuting - Jiangnan University|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/4/2017
Publication Date: N/A
Technical Abstract: Whether for understanding the properties of food allergens or for manufacturing vaccines for allergen-specific immunotherapy, well characterized pure allergens are required. This often necessitate the use of recombinant technology in obtaining food allergens due to the very low amounts of their natural sources. In addition, recombinant expression of food allergens is also required when site-directed mutagenesis technologies is required in studying the allergenicity of food allergens. Bacterial expression systems are wildly used for protein expression because of its ease of use compared to other expression systems. Unfortunately, expressing food allergens in bacteria often meets with difficulty when the bacteria do not express the foreign proteins. Here we report the characterization of food allergens produced with new expression vectors.