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ARS Home » Crop Production and Protection » Research » Research Project #440135

Research Project: Barley Exosome Cataloging by Proteomics and RNAseq

Location: Crop Production and Protection

Project Number: 0500-00053-005-134-G
Project Type: Grant

Start Date: May 1, 2022
End Date: Apr 30, 2025

1. Improve exosome purification via size exclusion chromatograph/RNase/protease treatments. 2. Catalog/characterize exosomal sRNAs and proteins from exosomes from control and treated (FHB/trichothecene) barley seedlings. 3. Use super resolution microscopy to image exosomes and measure impact of isolated exosomes on fungal growth (in vitro).

We have successfully isolated barley exosomes using ultracentrifugation and density gradients. Our previous proteomic analysis indicated that additional purification (size exclusion chromatography and/or RNase/proteinase treatment) is necessary to improve the quality of our proteomic and genomics results. We have all the tools needed to meet the three objectives listed above.