Location: Crop Production and Protection
Project Number: 0500-00053-005-134-G
Project Type: Grant
Start Date: May 1, 2022
End Date: Apr 30, 2025
Objective:
1. Improve exosome purification via size exclusion chromatograph/RNase/protease treatments.
2. Catalog/characterize exosomal sRNAs and proteins from exosomes from control and treated (FHB/trichothecene) barley seedlings.
3. Use super resolution microscopy to image exosomes and measure impact of isolated exosomes on fungal growth (in vitro).
Approach:
We have successfully isolated barley exosomes using ultracentrifugation and density gradients. Our previous proteomic analysis indicated that additional purification (size exclusion chromatography and/or RNase/proteinase treatment) is necessary to improve the quality of our proteomic and genomics results. We
have all the tools needed to meet the three objectives listed above.
