Research Project: Intervention Strategies to Control Salmonella and Campylobacter During Poultry Processing

Location: Poultry Microbiological Safety and Processing Research Unit

Publications (Clicking on the reprint icon will take you to the publication reprint.)

In-vitro synergistic effects of peracetic acid and biocides approved for use in poultry meat processing against four serotypes of non-typhoidal Salmonella enterica - (Peer Reviewed Journal)
Mcmillan, E.A., Read, Q.D., Mitchell, T.R., Harris, C.E., Glenn, A.E., Pokoo-Aikins, A., Meinersmann, R.J., Frye, J.G. 2025. In-vitro synergistic effects of peracetic acid and biocides approved for use in poultry meat processing against four serotypes of non-typhoidal Salmonella enterica. Microbiology Spectrum. https://doi.org/10.1128/spectrum.00652-25.

Genotypic and phenotypic characterization of antimicrobial resistance in Salmonella strains isolated from both no-antibiotics-ever (NAE) and conventional broiler complexes - (Peer Reviewed Journal)
Adhikari, Y., Bourassa, D.V., Poudel, S., Bailey, M.A., Buhr, R.J., Macklin, K.S. 2025. Genotypic and phenotypic characterization of antimicrobial resistance in Salmonella strains isolated from both no-antibiotics-ever (NAE) and conventional broiler complexes. Poultry Science. 104(3):104855. https://doi.org/10.1016/j.psj.2025.104855.

Draft genome sequences of fifty-eight isolates of Campylobacter jejuni and Campylobacter coli from domestic ducks - (Peer Reviewed Journal)
Meinersmann, R.J., Robinson-Mckenzie, T.S., Mcmillan, E.A. 2025. Draft genome sequences of fifty-eight isolates of Campylobacter jejuni and Campylobacter coli from domestic ducks. Microbiology Resource Announcements. e00903-24. https://doi.org/10.1128/mra.00903-24.

Synergistic effects of polyphenols and antibiotics against multi-drug resistant salmonella typhimurium DT104 - (Abstract Only)
Sheffield, H., Rodriguez Chinchilla, K.E., Bharathan, G., Hayden, M., Madi, A., Buhr, R.J., Srikumar, S. 2025. Synergistic effects of polyphenols and antibiotics against multi-drug resistant salmonella typhimurium DT104. International Poultry Scientific Forum. 104:34.

Ferric Uptake Regulator (Fur) plays a significant role in the survival of Salmonella Typhimurium on meat - (Abstract Only)
Bharathan, G., Chinchilla, K.E., Sheffield, H., Hayden, M., Madi, A., Buhr, R.J., Srikumar, S. 2025. Ferric Uptake Regulator (Fur) plays a significant role in the survival of Salmonella Typhimurium on meat. International Poultry Scientific Forum. 104(E.Suppl.1): 33.

Synergistic Effects of Polyphenols and Antibiotics Against Multi-Drug Resistant Salmonella Typhimurium DT104 - (Abstract Only)
Sheffield, H., Chinchilla, K.E., Bharathan, G., Hayden, M., Madi, A., Buhr, R.J., Srikumar, S. 2025. Synergistic Effects of Polyphenols and Antibiotics Against Multi-Drug Resistant Salmonella Typhimurium DT104. International Poultry Scientific Forum. 104(E.Suppl.1):p.34.

Evaluating the efficacy of a novel two-sided drop-through photonic decontamination system on Salmonella reduction - (Abstract Only)
Mcconnell, A., Riggs, M., Manjankattil, S., Poudel, S., Jennings, M., Hughes, M., Rawson, I., Buhr, R.J., Bourassa, D.V. 2024. Evaluating the efficacy of a novel two-sided drop-through photonic decontamination system on Salmonella reduction. International Poultry Scientific Forum. 103(E-Suppl.1):p.10.

A longitudinal study on Campylobacter spp. in an integrated broiler complex of south-east region of the United States: Prevalence and phylogenetic analysis - (Peer Reviewed Journal)
Adhikari, Y., Bailey, M., Bourassa, D.V., Poudel, S., Buhr, R.J., Macklin, K.S. 2025. A longitudinal study on Campylobacter spp. in an integrated broiler complex of south-east region of the United States: Prevalence and phylogenetic analysis. Journal of Food Protection. 88(1): 1-13.

Sixty-five draft genome sequences of Campylobacter coli and Campylobacter jejuni from table egg layer chickens - (Peer Reviewed Journal)
Meinersmann, R.J., Robinson-Mckenzie, T.S., Garcia, J.S., Jones, D.R. 2024. Sixty-five draft genome sequences of Campylobacter coli and Campylobacter jejuni from table egg layer chickens. Microbiology Resource Announcements. 0:e00670-24. https://doi.org/10.1128/mra.00670-24.

Assessment and genetic analysis of Salmonella and Campylobacter from various stages of an integrated No-Antibiotics-Ever (NAE) broiler complex - (Peer Reviewed Journal)
Adhikari, Y., Bailey, M.A., Krehling, J.T., Kitchens, S., Gaonkar, P., Munoz, L.R., Escobar, C., Buhr, R.J., Laura, H., Price, S.B., Bourassa, D.V., Macklin, K.S. 2024. Assessment and genetic analysis of Salmonella and Campylobacter from various stages of an integrated No-Antibiotics-Ever (NAE) broiler complex. Poultry Science. 103(11):104212. https://doi.org/10.1016/j.psj.2024.104212.

Susceptibility of pESI positive Salmonella to treatment with biocide chemicals approved for use in poultry processing as compared to Salmonella without the pESI plasmid - (Peer Reviewed Journal)
Mcmillan, E., Adams, E.S., Mitchell, T.R., Hawkins, J.A., Read, Q.D., Pokoo-Aikins, A., Meinersmann, R.J., Harris, C., Hughes Jr, M.D., Glenn, A.E., Berrang, M.E. 2024. Susceptibility of pESI positive Salmonella to treatment with biocide chemicals approved for use in poultry processing as compared to Salmonella without the pESI plasmid. Letters in Applied Microbiology. https://doi.org/10.1093/lambio/ovae067.

Feather retention during defeathering – impact of carcass rigor mortis. - (Abstract Only)
Harris, C.E., Buhr, R.J. 2024. Feather retention during defeathering – impact of carcass rigor mortis.. Poultry Science Association Meeting Abstract. 103(E.Suppl.1):624S, p.282.

Detection of Salmonella from pullet through the final raw product of a conventional broiler complex - (Abstract Only)
Adhikari, Y., Bailey, M.A., Gaonkar, P., Kitchens, S., Munoz, L.R., Bourassa, D.V., Price, S.B., Buhr, R.J., Macklin, K.S. 2024. Detection of Salmonella from pullet through the final raw product of a conventional broiler complex. Poultry Science Association Meeting Abstract. 103(E-Suppl.1): 59, pg.33.

Assessment of Campylobacter from production farms, hatchery, and processing plant of a conventional broiler complex - (Abstract Only)
Adhikari, Y., Bailey, M.A., Gaonkar, P., Kitchens, S., Munoz, L.R., Bourassa, D.V., Price, S.B., Buhr, R.J., Macklin, K.S. 2024. Assessment of Campylobacter from production farms, hatchery, and processing plant of a conventional broiler complex. Poultry Science Association Meeting Abstract. 103(E-Suppl.1: 527P, p. 244-245.

A Retrospective Analysis of Salmonella Isolates across 11 Animal Species (1982–1999) Led to the First Identification of Chromosomally Encoded blaSCO-1 in the USA - (Peer Reviewed Journal)
Iduu, N., Raiford, D., Conley, A., Scaria, J., Nelson, J., Ruesch, L., Price, S., Yue, M., Gong, J., Wei, L., Wang, C. 2024. A Retrospective Analysis of Salmonella Isolates across 11 Animal Species (1982–1999) Led to the First Identification of Chromosomally Encoded blaSCO-1 in the USA. Microorganisms. 12, 528. https://doi.org/10.3390/microorganisms12030528.

Reduction of bacterial load on broiler carcasses using low volume fluidic nozzles in combination with 60oC water at 450 psi pressure - (Peer Reviewed Journal)
Cosby, D.E., Mclntyre, M.D., Devoll, J., Jordan, A., Garrish, J.K., Berrang, M.E., Mcmillan, E.A. 2024. Reduction of bacterial load on broiler carcasses using low volume fluidic nozzles in combination with 60oC water at 450 psi pressure. Poultry. 3(1):15-25. https://doi.org/10.3390/poultry3010002.

Quantification of Salmonella Infantis transfer from transport drawer flooring to broiler chickens during holding - (Peer Reviewed Journal)
Reina, M.A., Mcconnell, A., Figueroa, J.C., Riggs, M.R., Buhr, R.J., Price, S.B., Macklin, K.S., Bourassa, D.B. 2023. Quantification of Salmonella Infantis transfer from transport drawer flooring to broiler chickens during holding. Poultry Science. 103(2). Article 10377. https://doi.org/10.1016/j.psj.2023.103277.

Application of pressurized steam and forced hot air for cleaning broiler transport container flooring - (Peer Reviewed Journal)
Reina, M.A., Urrutia, A., Figueroa, J.C., Riggs, M.R., Macklin, K.S., Buhr, R.J., Price, S.B., Bourassa, D.B. 2023. Application of pressurized steam and forced hot air for cleaning broiler transport container flooring. Poultry Science. 103(2). Article 103276. https://doi.org/10.1016/j.psj.2023.103276.

Impact of stunning method on blood loss in broilers during exsanguination with two different neck cut methods - (Peer Reviewed Journal)
Osborne, R.C., Harris, C.E., Buhr, R.J., Kiepper, B.H. 2023. Impact of stunning method on blood loss in broilers during exsanguination with two different neck cut methods. Journal of Applied Poultry Research. 31(1). Article 100385. https://doi.org/10.1016/j.japr.2023.100385.

Buffering capacity comparison of Tris Phosphate Carbonate (TPC) and buffered peptone water (BPW) Salmonella pre-enrichments for manufactured feed and feed ingredients - (Peer Reviewed Journal)
Escobar, C., Munoz, L.R., Bailey, M.A., Krehling, J.T., Pacheco, W.J., Hauck, R., Buhr, R.J., Macklin, K.S. 2023. Buffering capacity comparison of Tris Phosphate Carbonate (TPC) and buffered peptone water (BPW) Salmonella pre-enrichments for manufactured feed and feed ingredients. Poultry Science. 13(19):3119. https://doi.org/10.3390/ani13193119.

Prevalence and subtype characterization of Campylobacter in ceca of commercial broiler chickens at processing – a 452 flock, sevenyear surve - (Peer Reviewed Journal)
Meinersmann, R.J., Berrang, M.E., Mcmillan, E.A., Knapp, S.W. 2023. Prevalence and subtype characterization of Campylobacter in ceca of commercial broiler chickens at processing – a 452 flock, sevenyear surve. Journal of Food Protection. 86(100170). https://doi.org/10.1016/j.jfp.2023.100170.

In vitro Effect of photo-active compounds curcumin and chlorophyllin against salmonella and campylobacter - (Peer Reviewed Journal)
Urrutia, A., Orellana, L., Sierra, K.S., Reina, M.A., Figueroa, J.C., Jackson, A.P., Macklin, K.S., Buhr, R.J., Bourassa, D.V. 2023. Effect of photo-active compounds curcumin and chlorophyllin against Salmonella and Campylobacter. Journal of Food Protection. 86(11). https://doi.org/10.1016/j.jfp.2023.100157.

Role of darkling beetles (Alphitobius diaperinus) and litter in spreading and maintaining Salmonella Enteritidis and Campylobacter jejuni in chicken flocks - (Peer Reviewed Journal)

Recovery of Campylobacter injected into hatching eggs that are cold-stored and incubated through day 18 of incubation - (Abstract Only)
Harris, C.E., Bartenfeld Jossel, L.N., Buhr, R.J. 2023. Recovery of Campylobacter injected into hatching eggs that are cold-stored and incubated through day 18 of incubation. Poultry Science Association Meeting Abstract. 102(E-Suppl.1): 151. p74. 2023.

Exudate from retail chicken liver packaging allows for survival of naturally occurring Campylobacter, coliforms, and total aerobic bacteria under drying conditions - (Peer Reviewed Journal)
Adams, E.S., Mcmillan, E.A., Meinersmann, R.J., Berrang, M. 2023. Exudate from retail chicken liver packaging allows for survival of naturally occurring Campylobacter, coliforms, and total aerobic bacteria under drying conditions. Journal of Food Protection. 86(100123). https://doi.org/10.1016/j.jfp.2023.100123.

Head-to-head comparison of CAMPYAIR aerobic culture medium versus standard microaerophilic culture for Campylobacter isolation from clinical samples - (Peer Reviewed Journal)
Levican, A., Varela, C., Porte, L., Weitzel, T., Briceno, I., Guerra, F., Mena, B., Hinton Jr, A. 2023. Head-to-head comparison of CAMPYAIR aerobic culture medium versus standard microaerophilic culture for Campylobacter isolation from clinical samples. Frontiers in Cellular and Infection Microbiology. 13. https://doi.org/10.3389/fcimb.2023.1153693.

Assessment of Salmonella and Campylobacter among various stages of No-Antibiotics-Ever (NAE) commercial broiler complex. - (Abstract Only)
Adhikari, Y., Bailey, M., Krehling, J., Chasteen, K.S., Munoz, L.R., Lobo, C.E., Galindo, L.A., Gaonkar, P., Kitchens, S., Price, S., Bourassa, D.V., Buhr, R.J., Macklin, K.J. 2023. Assessment of Salmonella and Campylobacter among various stages of No-Antibiotics-Ever (NAE) commercial broiler complex.. Poultry Science Meeting. 102(E-Suppl.1): 149, p.73..

Association of Campylobacter from bile with cecal counts, gall bladder size, and bile color. - (Abstract Only)
Mcconnell, A., Jennings, M., Hughes, M., Figueroa, J., Reina, M.A., Buhr, R.J., Bourassa, D.V. 2023. Association of Campylobacter from bile with cecal counts, gall bladder size, and bile color.. Poultry Science Meeting. 102(E-Suppl.1):150, p.74.

Quantification of Salmonella Infantis transfer from transport drawer flooring to broiler chickens during different lairage times - (Abstract Only)
Reina, M., Mcconnell, A., Figueroa, J., Riggs, M., Jennings, M., Contreras, F., Hughes, M., Buhr, R.J., Bourassa, D. 2023. Quantification of Salmonella Infantis transfer from transport drawer flooring to broiler chickens during different lairage times. Poultry Science Association Meeting Abstract. 102(E-Suppl.1): 144, p.71..

Isolation and classification of bacteriophage sourced from poultry associated samples - (Abstract Only)
Figueroa, J.C., Kitchens, S., Price, S., Buhr, R.J., Bourassa, D.V. 2023. Isolation and classification of bacteriophage sourced from poultry associated samples. Poultry Science Association Meeting Abstract. 102(E-Suppl.1) 146, p. 72.

Role of darkling beetles (Alphitobius diaperinus) in spreading and maintaining Salmonella Enteritidis and Campylobacter jejuni in chicken flocks - (Abstract Only)
Barua, S., Bailey, M.A., Zhong, K., Iduu, N., Dormitorio, T., Macklin, K., Bourassa, D.V., Price, S., Hauck, R., Krehling, J., Kitchens, S., Kyriakis, C., Buhr, R.J., Wang, C. 2023. Role of darkling beetles (Alphitobius diaperinus) in spreading and maintaining Salmonella Enteritidis and Campylobacter jejuni in chicken flocks. Poultry Science Association Meeting Abstract. 102(E-Suppl.1): 288, p.139.

Isolation and identification of Salmonella and Campylobacter isolates from a commercial broiler complex through pullets to final raw product - (Abstract Only)
Adhikari, Y., Bailey, M., Krehling, J., Chasteen, K., Munoz, L., Lobo, C., Galindo, L., Gaonkar, P., Kitcherns, S., Bouradssa, D.V., Price, S., Buhr, R.J., Macklin, K. 2023. Isolation and identification of Salmonella and Campylobacter isolates from a commercial broiler complex through pullets to final raw product. International Poultry Scientific Forum. 102(E-Suppl.1): M8, p.4..

Application of pressurized steam and forced hot air for cleaning broiler transport cage flooring - (Abstract Only)
Reina, M.A., Urrutia, A., Figueroa, J., Riggs, M., Macklin, K., Buhr, R.J., Bourassa, D.V. 2023. Application of pressurized steam and forced hot air for cleaning broiler transport cage flooring. International Poultry Scientific Forum. 102(E-Suppl.1): M6, p.3..