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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Poultry Microbiological Safety and Processing Research Unit » Research » Publications at this Location » Publication #405421
Research Project: Intervention Strategies to Control Salmonella and Campylobacter During Poultry Processing

Location: Poultry Microbiological Safety and Processing Research Unit

Title: Buffering capacity comparison of Tris Phosphate Carbonate (TPC) and buffered peptone water (BPW) Salmonella pre-enrichments for manufactured feed and feed ingredients

Author
item ESCOBAR, CESAR - Auburn University
item MUNOZ, LUIS - Auburn University
item BAILEY, MATHEW - Auburn University
item KREHLING, JAMES - Auburn University
item PACHECO, WILMAR - Auburn University
item HAUCK, RUEDIGER - Auburn University
item Buhr, Richard
item MACKLIN, KENNETH - Auburn University

Submitted to: Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/4/2023
Publication Date: 10/6/2023
Citation: Escobar, C., Munoz, L.R., Bailey, M.A., Krehling, J.T., Pacheco, W.J., Hauck, R., Buhr, R.J., Macklin, K.S. 2023. Buffering capacity comparison of Tris Phosphate Carbonate (TPC) and buffered peptone water (BPW) Salmonella pre-enrichments for manufactured feed and feed ingredients. Poultry Science. 13(19):3119. https://doi.org/10.3390/ani13193119.
DOI: https://doi.org/10.3390/ani13193119

Interpretive Summary: Numerous methods to detect Salmonella in animal feed have been used due to the impact foodborne Salmonella has on public health. For this project, two buffers: tris phosphate carbonate (TPC) and buffered peptone water (BPW) were compared as pre-enrichment mediums for recovery of Salmonella in feed ingredients. A total of 269 samples were collected from 6 feed mills and mixed with the buffers and then initial pH was measured before and final after a 24 h incubation period. Differences were observed comparing pH values by feed sample type; distillers dried grain and poultry by-product meal presented lower initial pH values for TPC and BPW compared to the other samples. For both TPC and BPW buffers, meat and bone meal presented higher final pH values, while soybean

Technical Abstract: Various culture-based methods to detect Salmonella in animal feed have been developed due to the impact of this bacterium on public and animal health. For this project, tris phosphate carbonate (TPC) and buffered peptone water (BPW) buffering capacity were compared as pre-enrichment mediums for detection of Salmonella in feed ingredients. A total of 269 samples were collected from 6 feed mills and mixed with the pre-enrichments; pH was measured before and after a 24h incubation. Differences were observed comparing pH values by sample type, DDGS and poultry by-product meal presented lower initial pH values for TPC and BPW compared to the other samples. For both TPC and BPW, meat and bone meal presented higher final pH values, while soybean meal and peanut