|Matteri, Robert - Bob|
|Carroll, Jeffery - Jeff Carroll|
Submitted to: Animal Science Progress Report
Publication Type: Other
Publication Acceptance Date: 8/3/1998
Publication Date: N/A
Technical Abstract: The objective of this study was to evaluate the effects of recombinant growth hormone (GH) treatment and age on adipose tissue leptin gene expression in the early developmental period of pigs. Three groups of barrows were used in this study. Daily i.m. injections of GH (200 ug/kg) or vehicle were administered beginning at either 1, 4, or 9 wk of age (8 treated and 8 control animals/age group). Animals were sacrificed after 2 wk of treatment. Serum IGF-1 concentrations in GH vs control pigs sacrificed at 3, 6, and 11 wk of age were 83.5 vs 67.8, 197.6 vs 144.1, and 331.3 vs 225.1 ng/ml, respectively (LSE = 9.7; GH, P = .004; Age, P<.0001; GH X Age, P = .17). Growth was unaffected by GH treatment (P = .46). Percent body fat (11.3% overall average), evaluated by Dual-energy X-ray absorptiometry (DEXA), did not differ with age (P = .27) and was not affected by GH treatment (P = .14). Levels of GH receptor mRNA in adipose tissue increased with age (P = .0004), and were reduced in all age groups by GH treatment (P = .042). GH receptor and leptin mRNA levels were correlated in adipose tissue (r = .46, P = .001), however the quantity of leptin mRNA was not affected by GH treatment (P = .71). Leptin mRNA levels were not affected by age (P = .86).