Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/12/1995
Publication Date: N/A
Technical Abstract: Liquid nitrogen (-196 C) was used to preserve healthy and deteriorated cottonseed over various time periods from 72 hr to 90 days. The seeds were then returned to 25 C for several days and evaluated for quality, using the Cool-Warm Vigor Index test. Storage at -196 C had no significant effect on the seed germination and vigor of different varieties sof seed, but it often caused minor to severe cracks in the cotyledonary tissues. Typical seedlings, 10 to 25 mm in length, were planted in peat pellets and allowed to grow for 2 to 3 wk. They were then transferred to potted soil and allowed to develop into mature plants. The cotyledonary changes often slowed growth of the plants but only occasionally prevented seed from developing into adult plants capable of producing cotton. This suggests that the cryogenic method may be suitable for long term preservation of cottonseed germplasm.