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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #290568
Title: Storage proteins of common bean identified with 2D-PAGE

Author
item Natarajan, Savithiry
item Pastor Corrales, Marcial

Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Trade Journal
Publication Acceptance Date: 2/10/2013
Publication Date: 2/10/2013
Citation: Natarajan, S.S., Pastor Corrales, M.A. 2013. Storage proteins of common bean identified with 2D-PAGE. Bean Improvement Cooperative Annual Report. 56:15-17.

Interpretive Summary:

Technical Abstract: The common bean is a significant source of protein, complex carbohydrates, fiber, and minerals. Seeds of most dry beans contain 15 to 25% protein and are rich in lysine but low in the sulfur containing amino acids cysteine and methionine. Knowledge of common bean proteins is important for research aimed at obtaining common bean cultivars with value-added proteins. In this study, we used proteomic technologies including 2D-PAGE for protein separation and mass spectrometry for protein characterization. Our results showed that 29 protein spots were identified as alpha-phaseolin. In addition, two spots were identified as beta-phaseolin and 28 spots were identified as phaseolin. In addition, we found 21 protein spots that were identified as legumin and three protein spots as albumin-2 based upon our 2D-PAGE analysis. The identification of storage proteins of common bean in our study is useful to bean scientists endeavoring to develop common bean cultivars with value added proteins.