|KENERLEY, CHARLES - Texas A&M University|
|Stipanovic, Robert - Bob|
|MUKHERJEE, PRASUN - Texas A&M University|
|MORAN-DIEZ, MARIA - Texas A&M University|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/2011
Publication Date: 7/27/2011
Citation: Kenerley, C.M., Stipanovic, R.D., Mukherjee, P.K., Moran-Diez, M.E. 2011. Ability of secondary metabolites from trichoderma virens to mediate communication during mutualistic or pathogenic interactions. 2011 NRI/AFRI MIcrobial Biology and Microbial Functional Genomics Awardee Meeting, July 26-27, 2011, Washington, D.C. p55.
Technical Abstract: A bioinformatic study was conducted to identify the putative genes in the biocontrol agent Trichoderma virens that encode for non-ribosomal peptide synthetases (NRPS). Gene expression analysis of 22 putative NRPSs and 4 NRPS/PKS (polyketide synthase) hybrid enzymes was conducted in the presence and absence of roots in a hydroponic system, during growth on several media, and in confrontation with R. solani and T. virens itself. Based upon the gene expression studies, vectors were constructed to disrupt approximately half of these genes by PEG mediated transformation. Phenotypic analysis of the majority of these mutants has been completed. The mutants were assayed for growth, conidial production, hyphal and colony morphology, and conidial germination. Some genes have been characterized for their biosynthetic products (e.g., peptaibols, gliotoxin, and siderophores), ability to colonize roots and induce defense response in maize.