|Seabourn, Bradford - Brad|
|Xiao, Zhihua - Susan|
|Tilley, Michael - Mike|
|PARK, SEOKHO - Former ARS Employee|
Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/29/2011
Publication Date: 5/1/2012
Citation: Seabourn, B.W., Xiao, Z.S., Tilley, M., Herald, T.J. and Park, S. 2012. A rapid, small-scale sedimentation method to predict breadmaking quality of hard winter wheat. Crop Science. 52(3):1306-1315.
Interpretive Summary: Wheat gluten provides unique functional properties to bread dough in terms of end-use quality. Breeders and processors need tests that more rapidly and accurately evaluate wheat gluten quality. A small scale sedimentation method was developed to expedite the analysis time for predicting hard winter wheat protein quality. The method was developed by combining the sodium dodecyl-sulfate (SDS) sedimentation method (AACC 56-70) and solvent retention capacity (SRC) test method (AACC 56-11) to create a hybrid method that is both rapid and small scale. The new method was able to simultaneously test wheat samples in 66% less time compared to the AACC method and HWWQL sedimentation methods. Furthermore, sample size was reduced from 6 g for the AACC method to 1 g for the hybrid SDS-SRC method. In addition, the SDS-SRC exhibited a higher correlation to loaf volume compared to the HWWQL method. Due to enhanced speed, accuracy, and simplicity, the Hybrid SRC-SDS sedimentation method may prove useful in breeding programs and other scenarios where rapid assessment of end-use quality determinations is required.
Technical Abstract: Breeders and processors are always looking for rapid and accurate methods to evaluate wheat quality. A rapid small-scale hybrid sedimentation method was developed for predicting breadmaking quality of breeders samples by combining the sodium dodecyl-sulfate (SDS) sedimentation method (AACC 56-70) and solvent retention capacity (SRC) test method (AACC 56-11). A preliminary test of the hybrid SDS-SRC method was performed on eight hard winter wheat (HWW) varieties and compared to AACC (56-70) and in-house Hard Winter Wheat Quality Laboratory (HWWQL) methods to show proof of concept. Further validation of the hybrid method was conducted on a diverse set of 53 HWW varieties. The hybrid method was performed in 66% less time than AACC Method 56-70 and HWWQL methods. Furthermore, sample size was reduced from 6 g for the AACC method to 1 g for the hybrid method. The hybrid method exhibited a higher correlation to bread loaf volume (r = 0.84) compared to the AACC method (r > 0.42) and HWWQL method (r = 0.64) for wheat flour. Due to enhanced speed, accuracy, and simplicity, the hybrid SDS-SRC sedimentation method may prove useful in breeding programs and other scenarios where rapid assessment of end-use quality determination is required.