Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/3/2010
Publication Date: 8/31/2010
Citation: Bronstein, P., Butcher, B.G., Myers, C.R., Stodghill, P., Swingle, B.M., Cartinhour, S.W. 2010. Global survey of Fur binding refines the iron responsive regulon of Pseudomonas syringae [abstract]. p. 31. Interpretive Summary:
Technical Abstract: Pseudomonas syringae must sense and respond to a variety of environmental signals and understanding how the bacterium integrates these signals into a physiological response is central to our understanding of this plant pathogen. One important micronutrient for all biological organisms is iron. Previously we studied the transcriptional response of P. syringae pv tomato DC3000 (DC 3000) to levels of bioavailable iron. A major player in the transcription response to bioavailable iron in many bacteria is the Ferric Uptake Regular, Fur. To define specific regions where Fur binds in the DC3000 genome we combined chromatin immunoprecipitation assays with high throughput sequencing (ChIP-seq). After mapping the enriched DNA sequences from the immunoprecipitation to the DC3000 genome we identified many putative Fur binding sites. These sites included regions upstream of known Fur targets, other iron-repressed genes, iron-induced genes, and several known or putative regulatory genes. Additionally, we used DNaseI footprinting to confirm many putative Fur binding sites derived from the ChIP-seq data set. This study has allowed us to refine the DC3000 iron-responsive regulon and infer the primary regulation of specific transcripts by Fur.