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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Emerging Pests and Pathogens Research » People & Locations » Bryan Swingle

Bryan M Swingle
Emerging Pests and Pathogens Research
Research Molecular Biologist

Phone: (607) 255-7894
Fax: (607) 255-4471
Room 302

RW HOLLEY CTR
538 TOWER RD
ITHACA, NY 148532901
Projects
Genomic and Functional Analysis of Emerging and Persistent Plant Pathogenic Bacteria to Support Disease Control
Cooperative Agreement (A)
  Accession Number: 433486
Genomics Approaches for Understanding and Managing Blackleg and Soft Rot in Potato
Cooperative Agreement (A)
  Accession Number: 434607
Characterization of Molecular Networks in Diseases Caused by Emerging and Persistent Bacterial Plant Pathogens
In-House Appropriated (D)
  Accession Number: 432528
Integrating Next-generation Technologies for Blackleg and Soft Rot Management in Potato
Reimbursable Cooperative Agreement (R)
  Accession Number: 433967
Identifying Novel Potato Germplasm with Resistance Against Cyst Nematode and Dickeya Pathogens
Non-Assistance Cooperative Agreement (S)
  Accession Number: 433687
Identifying Novel Potato Germplasm with Resistance Against Cyst Nematode and Dickeya Pathogens
Non-Assistance Cooperative Agreement (S)
  Accession Number: 435578

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Complete genome sequence of a gram positive bacterium Leifsonia sp. strain PS1209, a potato endophyte Reprint Icon - (Peer Reviewed Journal)
Liu, Y., Ma, X., Helmann, T.C., Mclane, H., Stodghill, P., Swingle, B.M., Filiatrault, M.J., Perry, K.L. 2020. Complete genome sequence of a gram positive bacterium Leifsonia sp. strain PS1209, a potato endophyte. Microbiology Resource Announcements. 9(26). https://doi.org/10.1128/MRA.00447-20.
Pseudomonas syringae AlgU downregulates flagellin gene expression helping evade plant immunity Reprint Icon - (Peer Reviewed Journal)
Swingle, B.M., Bao, Z., Wei, H., Ma, X. 2020. Pseudomonas syringae AlgU downregulates flagellin gene expression helping evade plant immunity. Journal of Bacteriology. https://doi.org/10.1128/JB.00418-19.
First report of Dickeya fangzhongdai causing onion soft rot in New York State Reprint Icon - (Peer Reviewed Journal)
Swingle, B.M., Ma, X., Bonasera, J.M., Asselin, J., Beer, S.V. 2020. First report of Dickeya fangzhongdai causing onion soft rot in New York State. Plant Disease. 104(4):1251-1252. https://doi.org/10.1094/PDIS-09-19-1940-PDN.
Complete genome sequence of Dickeya dianthicola ME23, a pathogen causing Blackleg and Soft Rot Diseases of potato Reprint Icon - (Peer Reviewed Journal)
Swingle, B.M., Perna, N.T., Glasner, J.D., Hao, J., Johnson, S., Charkowski, A., Perry, K.L., Stodghill, P. 2019. Complete genome sequence of the potato blackleg pathogen Dickeya dianthicola ME23. Microbiology Resource Announcements. 8(7). https://doi.org/10.1128/MRA.01526-18.
First report of Rhizopus oryzae causing Potato Soft Rot in the Hebei Province of China Reprint Icon - (Peer Reviewed Journal)
Swingle, B.M., Cui, W.G., Zheng, H.L., Zhang, F.B., Zhu, H.T., Gao, M. 2019. First report of Rhizopus oryzae causing Potato Soft Rot in the Hebei Province of China. Plant Disease. 104. https://doi.org/10.1094/PDIS-09-18-1612-PDN.
Pectobacterium and Dickeya responsible for potato blackleg disease in New York state in 2016 Reprint Icon - (Peer Reviewed Journal)
Ma, X., Perry, K., Swingle, B.M., Perry, K. 2018. Pectobacterium and Dickeya responsible for potato blackleg disease in New York state in 2016. Plant Disease. 102(9):1834-1840. https://doi.org/10.1094/PDIS-10-17-1595-RE.
An AlgU-regulated antisense transcript encoded within the Pseudomonas syringae fleQ gene has a positive effect on motility Reprint Icon - (Peer Reviewed Journal)
Markel, E.J., Wall, H., Monteil, C., Vinatzer, B., Swingle, B.M. 2018. An AlgU-regulated antisense transcript encoded within the Pseudomonas syringae fleQ gene has a positive effect on motility. Journal of Bacteriology. https://doi.org/10.1128/JB.00576-17.
The ECF sigma factor, PSPTO_1043, in Pseudomonas syringae pv. tomato DC3000 is induced by oxidative stress and regulates genes involved in oxidative stress response Reprint Icon - (Peer Reviewed Journal)
Butcher, B., Bao, Z., Wilson, J., Stodghill, P., Swingle, B.M., Filiatrault, M.J., Schneider, D.J., Cartinhour, S.W. 2017. The ECF sigma factor, PSPTO_1043, in Pseudomonas syringae pv. tomato DC3000 is induced by oxidative stress and regulates genes involved in oxidative stress response. PLoS One. DOI: 10.1371/journal.pone.0180340.
Testing for Blackleg and Dickeya in the 2017 field season ()
Swingle, B.M., Perry, K. 2017. Testing for Blackleg and Dickeya in the 2017 field season. Empire State Potato Growers (Newsletter). Available: http://empirepotatogrowers.com/testing-for-blackleg-and-dickeya-in-the-2017-field-season/.
Comparative genomics of pseudomonas syringae pathovar tomato reveals novel chemotaxis pathways associated with motility and plant pathogenicity - (Peer Reviewed Journal)
Clarke, C.R., Hayes, B.W., Runde, B.J., Markel, E.J., Webb, B.A., Scharf, B.E., Swingle, B.M., Vinatzer, B.A. 2016. Comparative genomics of pseudomonas syringae pathovar tomato reveals novel chemotaxis pathways associated with motility and plant pathogenicity. PeerJ. 4:e2570.
Population genomic insights into the emergence, crop-adaptation and dissemination of Pseudomonas syringae pathogens - (Peer Reviewed Journal)
Swingle, B.M., Monteil, C.L., Yahara, K., Studholme, D.J., Mageiros, L., Meric, G., Morris, C.E., Vinatzer, B.A., Sheppard, S.K. 2016. Population genomic insights into the emergence, crop-adaptation and dissemination of Pseudomonas syringae pathogens. PLoS Pathogens. 2(10):e000089.
AlgU controls expression of virulence genes in Pseudomonas syringae pv. tomato DC3000 - (Peer Reviewed Journal)
Markel, E.J., Stodghill, P., Bao, Z., Myers, C., Swingle, B.M. 2016. AlgU controls expression of virulence genes in Pseudomonas syringae pv. tomato DC3000. Journal of Bacteriology. 198(17):2330-2344.
Pseudomonas syringae pv. Tomato DC3000 Type III secretion effector polymutants reveal an interplay between hopAD1 and AvrPtoB - (Peer Reviewed Journal)
Wei, H., Chakravarthy, S., Mathieu, J., Helmann, T.C., Stodghill, P., Swingle, B.M., Martin, G.B., Collmer, A. 2015. Pseudomonas syringae pv. Tomato DC3000 Type III secretion effector polymutants reveal an interplay between hopAD1 AvrPtoB. Cell Host and Microbe. 17(6):752-62.
Global analysis of the HrpL regulon in the plant pathogen Pseudomonas syringae pv. tomato DC3000 reveals new regulon members with diverse functions - (Peer Reviewed Journal)
Lam, H.N., Chakravarthy, S., Wei, H., Buinguyen, H., Stodghill, P., Swingle, B.M., Collmer, A., Cartinhour, S.W. 2014. Global analysis of the HrpL regulon in the plant pathogen Pseudomonas syringae pv. tomato DC3000 reveals new regulon members with diverse functions. PLoS Pathogens. 9(8):e106115. DOI: 10.1371/journal.pone.0106115.
Genomic plasticity enables phenotypic variation of Pseudomonas syringae pv. tomato DC3000 - (Peer Reviewed Journal)
Swingle, B.M., Bao, Z., Stodghill, P., Myers, C.R., Lam, H., Markel, E.J., Collmer, A., Cartinhour, S.W., Schweitzer, P. 2014. Genomic plasticity enables phenotypic variation of Pseudomonas syringae pv. tomato DC3000. PLoS One. 9(2):e86628. DOI: 10.1371/Journal.pone.0086628.
RecTEPsy mediated recombineering in Pseudomonas syringae ()
Swingle, B.M. 2014. RecTEPsy mediated recombineering in Pseudomonas syringae. Methods in Molecular Biology. 1114:3-10. DOI: 10.1007/978-1-62703-761-7_1.
Oligonucleotide recombination enabled site-specific mutagenesis in bacteria ()
Swingle, B.M. 2013. Oligonucleotide recombination enabled site-specific mutagenesis in bacteria. Methods in Molecular Biology. 978:127-132.
Pseudomonas syringae pv. tomato DC3000 CmaL (PSPTO4723), a DUF1330 family member, is needed to produce L-allo-isoleucine, a precursor for the phytotoxin coronatine - (Peer Reviewed Journal)
Worley, J.N., Russell, A.B., Wexler, A.G., Bronstein, P., Kvitko, B.H., Krasnoff, S., Munkvold, K.R., Swingle, B.M., Gibson, D.M., Collmer, A. 2013. Pseudomonas syringae pv. tomato DC3000 CmaL (PSPTO4723), a DUF1330 family member, is needed to produce L-allo-isoleucine, a precursor for the phytotoxin coronatine. Journal of Bacteriology. 195(2):287-296.
Regulons of the Pseudomonas syringae pv. tomato DC3000 iron starvation sigma factors PSPTO_0444, PSPTO_1209 and PSPTO_1286 - (Peer Reviewed Journal)
Swingle, B.M., Markel, E.J., Butcher, B.G., Myers, C.R., Stodghill, P., Cartinhour, S.W. 2013. Regulons of the Pseudomonas syringae pv. tomato DC3000 iron starvation sigma factors PSPTO_0444, PSPTO_1209 and PSPTO_1286. Applied and Environmental Microbiology. 79(2):725-727.
Substrate and target sequence lengths influence RecTEPsy recombineering efficiency in Pseudomonas syringae - (Peer Reviewed Journal)
Swingle, B.M., Bao, Z., Cartinhour, S.W. 2012. Substrate and target sequence lengths influence RecTEPsy recombineering efficiency in Pseudomonas syringae. PLoS One. 7(11):e50617. DOI: 10.1371/journal.pone.0050617.
An ECF sigma factor mediated cell surface signaling system in Pseudomonas syringae pv. tomato DC3000 regulates gene expression in response to heterologous siderophores - (Peer Reviewed Journal)
Swingle, B.M., Markel, E.J., Maciak, C.L., Butcher, B., Myers, C., Stodghill, P., Cartinhour, S.W., Bao, Z. 2011. An ECF sigma factor mediated cell surface signaling system in Pseudomonas syringae pv. tomato DC3000 regulates gene expression in response to heterologous siderophores. Journal of Bacteriology. 193:5775-5783.
New strategies for genetic engineering Pseudomonas syringae using recombination - (Abstract Only)
Swingle, B.M. 2011. New strategies for genetic engineering Pseudomonas syringae using recombination. Molecular Genetics of Bacteria and Phage. p. 20.
Characterization of the Fur regulon in Pseudomonas syringae pv. tomato DC3000 - (Peer Reviewed Journal)
Butcher, B.G., Bronstein, P., Myers, C., Stodghill, P., Bolton, J.J., Markel, E.J., Filiatrault, M.J., Swingle, B.M., Gaballa, A., Helmann, J.D., Schneider, D.J., Cartinhour, S.W. 2011. Characterization of the Fur regulon in Pseudomonas syringae pv. tomato DC3000. Journal of Bacteriology. 193(18):4598-4611. Available: http://www.ncbi.nlm.nih.gov/pubmed/21784947.
Bacterial effector HopF2 interacts with AvrPto and suppresses Arabidopsis innate immunity at the plasma membrane - (Peer Reviewed Journal)
Wu, S., Lu, D., Kabbage, M., Wei, H., Swingle, B.M., Dickman, M., He, P., Shan, L. 2011. Bacterial effector HopF2 interacts with AvrPto and suppresses Arabidopsis innate immunity at the plasma membrane. Molecular Plant-Microbe Interactions. 24(5):585-593.
Recombineering Pseudomonas syringae - (Abstract Only)
Swingle, B.M. 2010. Recombineering Pseudomonas syringae [abstract]. The 8th International Conference on Pseudomonas Syringae and Related Pathogens. 8/31.
Global survey of Fur binding refines the iron responsive regulon of Pseudomonas syringae - (Abstract Only)
Bronstein, P., Butcher, B.G., Myers, C.R., Stodghill, P., Swingle, B.M., Cartinhour, S.W. 2010. Global survey of Fur binding refines the iron responsive regulon of Pseudomonas syringae [abstract]. p. 31.
Functional analysis of PSPTO_1203 a FecI-like ECF Sigma Factor of Pseudomonas syringae pv. tomato DC3000 - (Abstract Only)
Swingle, B.M. 2010. Functional analysis of PSPTO_1203 a FecI-like ECF Sigma Factor of Pseudomonas syringae pv. tomato DC3000. Gordon Research Conference Proceedings. 99/12.
Recombineering using RecTE from Pseudomonas syringae - (Peer Reviewed Journal)
Swingle, B.M., Bao, Z., Markel, E.J., Chambers, A., Cartinhour, S.W. 2010. Recombineering using RecTE from Pseudomonas syringae. Applied and Environmental Microbiology. 76(15):4960-4968.
Recombineering using RecET from Pseudomonas syringae - (Abstract Only)
Swingle, B.M., Bao, Z., Markel, E.J., Cartinhour, S.W. 2010. Recombineering using RecET from Pseudomonas syringae. Molecular Genetics of Bacteria and Phage. 61/34.
Oligonucleotide recombination: a hidden treasure - (Peer Reviewed Journal)
Swingle, B.M., Markel, E.J., Cartinhour, S.W. 2010. Oligonucleotide recombination: a hidden treasure. Bioengineered Bugs. 1(4):1-4.
A new shuttle vector for gene expression in biopolymer-producing Ralstonia eutropha - (Peer Reviewed Journal)
Solaiman, D., Swingle, B.M., Ashby, R.D. 2010. A new shuttle vector for gene expression in biopolymer-producing Ralstonia eutropha. Journal of Microbiological Methods. 82:120-123.
Isolation of novel Pseudomonas syringae promoters and functional characterization in polyhydroxyalkanoate-producing pseudomads - (Peer Reviewed Journal)
Solaiman, D., Swingle, B.M. 2010. Isolation of novel Pseudomonas syringae promoters and functional characterization in polyhydroxyalkanoate-producing pseudomads. New Biotechnology. 27(1):1-9.
Oligonucleotide recombination in gram negative bacteria - (Peer Reviewed Journal)
Swingle, B.M., Markel, E.J., Costantino, N., Bubunenko, M., Cartinhour, S.W., Court, D. 2010. Oligonucleotide recombination in gram negative bacteria. Molecular Microbiology. 75(1):138-148.
Oligonucleotide recombination in bacteria - (Abstract Only)
Swingle, B.M., Costantino, N., Court, D., Bubunenko, M.G., Cartinhour, S.W. 2009. Oligonucleotide recombination in bacteria. [abstract}. 17:28.
Oligo Recombination in Gram Negative Bacteria - (Abstract Only)
Swingle, B.M., Cartinhour, S.W., Court, D. 2009. Oligo Recombination in Gram Negative Bacteria. Molecular Genetics of Bacteria and Phage. p. 166.
ECF Sigma Factor regulation in Pseudomonas syringae - (Abstract Only)
Swingle, B.M. 2008. Ecf sigma factor regulation in pseudomonas syringae. Molecular Genetics of Bacteria and Phage. p. 139.
Characterization of Promoter Motifs Regulated by ECF Sigma Factors of Pseudomonas syringae - (Abstract Only)
Swingle, B.M. 2008. Characterization of Promoter Motifs Regulated by ECF Sigma Factors of Pseudomonas syringae. Gordon Research Conference Proceedings. 4-2:p.14.
Characterization of the Promoter Motif Regulated by PSPTO_1209 a FecI-like ECF Sigma Factor of Pseudomonas syringae pv. tomato DC3000 - (Abstract Only)
Swingle, B.M., Schneider, D.J. 2008. Characterization of the Promoter Motif Regulated by PSPTO_1209 a FecI-like ECF Sigma Factor of Pseudomonas syringae pv. tomato DC3000. American Society for Microbiology. H-039:p.128.
Characterization of the PvdS-regulated promoter motif in Pseudomonas syringae pv. tomato DC3000 reveals regulon members and insights regarding PvdS function in other pseudomonads - (Peer Reviewed Journal)
Swingle, B.M., Thete, D., Moll, M., Myers, C., Schneider, D.J., Cartinhour, S.W. 2008. Characterization of the PvdS-regulated promoter motif in Pseudomonas syringae pv. tomato DC3000 reveals regulon members and insights regarding PvdS function in other pseudomonads. Molecular Microbiology. 68(4):871-889.
Extracytoplasmic Function (ECF) Sigma Factor Gene Regulation in Pseudomonas syringae: Integrated Molecular and Computational Characterization of PvdS-Regulated Promoters - (Abstract Only)
Swingle, B.M., Thete,, D., Moll, M., Myers,, C., Schneider, D.J., Cartinhour, S.W. 2007. Extracytoplasmic Function (ECF) Sigma Factor Gene Regulation in Pseudomonas syringae: Integrated Molecular and Computational Characterization of PvdS-Regulated Promoters. Molecular Genetics of Bacteria and Phage. p. 262.
Pyoverdine and beyond: PvdS dependent gene regulation in Pseudomonas syringae - (Abstract Only)
Swingle, B.M., Thete,, D., Moll, M., Myers,, C., Schneider, D.J., Cartinhour, S.W. Pyoverdine and beyond: PvdS dependent gene regulation in Pseudomonas syringae. American Society for Microbiology Conference. 159A:p. 90.