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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Food Composition and Methods Development Laboratory » Research » Publications at this Location » Publication #240692

Title: Dual Parallel Mass Spectrometry (LC1/MS2 and LC2/MS2) for Lipid and Vitamin D Analysis

item Byrdwell, W Craig

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/13/2009
Publication Date: 5/13/2009
Citation: Byrdwell, W.C. Dual Parallel Mass Spectrometry (LC1/MS2 and LC2/MS2) for Lipid and Vitamin D Analysis. Meeting Abstract. American Oil Chemists’ Society, Orlando, Florida, May 3-6, 2009.

Interpretive Summary:

Technical Abstract: Atmospheric pressure chemical ionization (APCI) mass spectrometry (MS) and electrospray ionization (ESI) MS are complementary techniques that provide different types of information for lipids such as triacylglycerols (TAGs), phospholipids, and fat-soluble vitamins. Since no one technique is by itself ideal, we routinely employ two mass spectrometers in parallel to provide APCI-MS and ESI-MS, MS/MS and MSn data. Dual parallel mass spectrometers have been attached to the same chromatographic system to provide two or more types of information from components in the same column effluent, referred to as an LC1/MS2 approach. Alternatively, two chromatographic systems have been attached in a column-switching configuration to perform a total lipid analysis of both polar and non0polar lipid on normal-phase (NP) and reversed-phase (RP) systems, respectively, with detection by two different mass spectrometers simultaneously operated with different ionization modes. This is referred to as an LC2/MS2 arrangement. An LC1/MS2 approach has also been used for vitamin D analysis in foods, which allows accurate quantification using APCI-MS in SIM mode with parallel acquisition of APCI-MS for qualitative monitoring of interfering species.