|Stipanovic, Robert - Bob|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/16/2009
Publication Date: 3/20/2009
Citation: Golubenko, Z., Akhunov, A.A., Vshivkov, S.O., Pshenichnov, E.A., Namazov, S.E., Stipanovic, R.D. 2009. Quantitative determination of gossypol enantiomers using capillary zone electrophoresis of selected cotton cultivars possess high level of (+)-gossypol. Fifth International Congress Biotechnology, March 16-20, 2009, Moscow, Russia. p52.
Technical Abstract: As known, cotton oil-seed meal provides a high quality protein that is a valuable feed product for animal industries. However, its use is limited by the presence of a toxic compound called gossypol. This compound occurs in two enantiomeric forms that are designated as (+)- or (-)-gossypol; these enantiomers possess different toxicity. It has been established that cottonseed with high quantity of (+)-gossypol is not toxic and can be used as feed for non-ruminant animals. During several years we have been carrying out plant selections to develop cotton cultivar with a high level of (+)-gossypol in seed and with optimal agronomic valuable traits. These cotton cultivars are not transgenic and thus the protein obtained from its seeds is not a genetically modified product. To guide the breeding and selection process, quantitative analysis of gossypol enantiomers in flower petals and seeds was determined by HPLC. This method requires expensive accessories and reagents. We have developed for the first time a method to quantitate gossypol enantiomers using capillary zone electrophoresis. This method possesses benefits such as simplicity, availability, short analysis time and requires minimal amounts of samples and reagents. This technique can be widely used in different branches of analytical chemistry.