Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/31/2007
Publication Date: 7/31/2004
Publication URL: acs.confex.com/acs/rmrm07/techprogram/P46186.htm
Citation: 2004. On-probe atmospheric pressure thermal desorption-ESI-MS for the analysis of volatile analytes or volatile pryolysis products. Meeting Abstract. Online. Interpretive Summary:
Technical Abstract: An on-probe pyrolyzer has been constructed and interfaced with desorption electrospray ionization (DESI) mass spectrometry (MS) for the rapid analysis of non-volatile pyrolysis products. The detection and analysis of non-volatile pyrolysis products of peptides, proteins and the synthetic polymer poly(ethylene glycol) were demonstrated with this instrument. The on-probe pyrolyzer can be operated off-line or on-line with the DESI source and was interfaced with a tandem MS (MS/MS) instrument, which allowed for structure characterization of the non-volatile pyrolytic products. Advantages of this system are its simplicity and speed of analysis since the pyrolysis is performed in situ on the DESI source probe and hence, it avoids extraction steps and/or the use of matrices (e.g., as in MALDI–MS analyses).