Author
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Mecham, James |
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Submitted to: Meeting Abstract
Publication Type: Proceedings Publication Acceptance Date: 9/22/2006 Publication Date: N/A Citation: N/A Interpretive Summary: Bluetongue virus (BTV) infects sheep, cattle and other ruminants and is transmitted by Culicoides spp. of biting midges. Cell lines derived from C. sonorensis have been developed at the Arthropod-borne Animal Diseases Research Laboratory. These cell lines have been shown to support BTV replication, and are potentially valuable tools for better understanding virus replication in the insect vector. However, since little or no cytopathology is noted following infection, detection of virus in these insect cells requires indirect methods, such as co-cultivation with susceptible mammalian cell culture. This report describes the use of immunoperoxidase staining, enzyme-linked immunosorbent assay and In situ fluorescent staining to directly detect and quantitate BTV in infected Culicoides cell lines. These assays should facilitate the use of the insect cell lines for BTV isolation and studies of virus replication. Technical Abstract: Bluetongue virus (BTV) infects sheep, cattle and other ruminants and is transmitted by Culicoides spp. of biting midges. Cell lines derived from C. sonorensis have been developed at the Arthropod-borne Animal Diseases Research Laboratory. These cell lines have been shown to support BTV replication, and are potentially valuable tools for better understanding virus replication in the insect vector. However, since little or no cytopathology is noted following infection, detection of virus in these insect cells requires indirect methods, such as co-cultivation with susceptible mammalian cell culture. This report describes the use of immunoperoxidase staining, enzyme-linked immunosorbent assay and In situ fluorescent staining to directly detect and quantitate BTV in infected Culicoides cell lines. These assays should facilitate the use of the insect cell lines for BTV isolation and studies of virus replication. |
