Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 5/17/2006
Publication Date: 6/26/2006
Citation: Proszkowiec-Weglarz, M., Richards, M.P., Poch, S.M. 2006. Gallus gallus 5'-AMP-activated protein kinase beta-2 non-catalytic subunit transcript variant 3 (PRKAB2) mRNA, complete cds [abstract]. GenBank Accession Number DQ640762.
Technical Abstract: AMP-activated protein kinase (AMPK) is a sensor of cellular energy charge. Once activated by an increase in the AMP:ATP ratio, AMPK acts as a master switch to increase energy-generating and to reduce energy-consuming metabolic pathways. AMPK exists as an enzyme complex consisting of one catalytic subunit (alpha) and two regulatory subunits (beta and gamma). A molecular cloning strategy involving primer-directed reverse transcription polymerase chain reaction (RT-PCR) was developed to sequence 1671 base pairs (bp) of a chicken skeletal muscle-derived cDNA corresponding to the complete coding region and portions of the 5'-and 3'-untranslated regions of the chicken AMPK beta-2 mRNA transcript variant 3. Transcript variant 3 derives from the use of an alternate promoter and transcription initiation site and alternative splicing of exon 2 in the AMPK beta-2 gene that gives rise to an mRNA with a unique 5' end lacking sequence from exon 1. The chicken beta-2 transcript variant 3 mRNA codes for a predicted protein (272 amino acids) that is identical to the one derived from transcript variant 1. Also, there is evidence for the inclusion of an additional 6 nucleotides at the 3' end of exon 5 due to alternative splicing that gives rise to a modified transcript variant 3 in skeletal muscle tissue. The modified transcript 3 codes for a predicted 274 amino acid protein. This sequence data confirmed for the first time the existence and expression of the AMPK beta-2, transcript variant 3 mRNA in chickens. Such data will be useful to scientists interested in studying the structure and expression of the chicken AMPK beta-2 gene, as well as, in further defining the genetic basis and function of the AMPK pathway in poultry.