Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 5/17/2006
Publication Date: 6/26/2006
Citation: Proszkowiec-Weglarz, M., Richards, M.P., Poch, S.M. 2006. Gallus gallus 5'-AMP-activated protein kinase, beta-2 non-catalytic subunit, transcript variant 2, mRNA (PRKAB2), complete cds. [abstract]. GenBank Accession number DQ940761.
Technical Abstract: AMP-activated protein kinase (AMPK) serves a "fuel gauge" that responds to fluctuations in cellular energy level. AMPK exists as an enzyme complex consisting of one catalytic subunit (alpha) and two regulatory subunits (beta and gamma). A molecular cloning strategy involving primer-directed reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of 5' cDNA ends (5' RACE) was developed to sequence 1881 base pairs (bp) of a chicken liver-derived cDNA corresponding to the complete coding region and portions of the 5'- and 3'-untranslated regions of the chicken AMPK beta-2 mRNA transcript variant 2. Transcript variant 2 is produced by the use of an alternate promoter and transcription initiation site within the AMPK beta-2 gene that results in a unique 5' end for this mRNA that lacks sequence contained in exon 1. Additionally, alternative splicing of exon 5 leads to the insertion of 6 nucleotides at the 3'-end of exon 5. Transcript variant 2 containing the larger exon 5 appears to be the form predominantly expressed in liver tissue, however there is also evidence for expression of a modified transcript variant with a shortener (by 6 bp) exon 5 segment. The chicken AMPK beta-2 transcript variant 2 mRNA codes for a 274 amino acid protein that contains two extra amino acids at positions 180-181 as compared to the transcript variant 1-derived protein. This sequence data confirmed for the first time the existence and expression of the AMPK beta-2 variant 2 mRNA in chickens. Such data will be useful in studying the structure and expression of the chicken AMPK beta-2 gene, as well as, in further defining the function of the AMPK pathway in poultry.