|Compton, David - Dave|
|Li, Xin liang|
Submitted to: Great Lakes Regional American Chemical Society Symposium
Publication Type: Abstract only
Publication Acceptance Date: 6/2/2006
Publication Date: 6/2/2006
Citation: Laszlo, J.A., Compton, D.L., Li, X. 2006. Feruloyl esterase hydrolysis and recovery of ferulic acid from jojoba meal [abstract]. Proceedings of the Great Lakes Regional American Chemical Society Symposium. p. 125 Interpretive Summary:
Technical Abstract: There is growing interest in recovering ferulic acid from plant sources for use as feedstock for several high-value applications. The feruloyl esterase domain of the Clostridium thermocellum cellulosomal xylanase was employed to hydrolyze ferulic acid from defatted jojoba meal. Esterase treatment produced 6.7 g of ferulic acid per kg of jojoba meal. The predominant source of the ferulate was found to originate from the meal’s water-soluble simmondsin fraction. Seven feruloyl simmondsin species from jojoba meal were identified by liquid chromatography–mass spectroscopy. Only one species, a didemethylsimmondsin ferulate, displayed a turnover rate with the enzyme distinctly faster than the other feruloyl simmondsins. Complete hydrolysis of all feruloyl simmondsin species was achieved in 24-48 h with a 100:1 meal:enzyme weight ratio. Ferulic acid was efficiently recovered from the medium by ethyl acetate extraction. The recovered ferulic acid was readily converted to ethyl ferulate, demonstrating a facile procedure for producing a valuable product from defatted jojoba meal.