Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 2/13/2006
Publication Date: 3/6/2006
Citation: Proszkowiec-Weglarz, M., Richards, M.P., Poch, S.M. 2006. Gallus gallus 5'-AMP-activated protein kinase beta-1 non-catalytic subunit (PRKAB1) mRNA, complete cds. GenBank Accession Number DQ398947.
Technical Abstract: AMP-activated protein kinase (AMPK) is a sensor of cellular energy charge. Once activated by an increase in the AMP/ATP ratio, AMPK acts as a master switch to increase energy-generating and to reduce energy-consuming metabolic pathways. AMPK exists as an enzyme complex consisting of one catalytic subunit (alpha) and two regulatory subunits (beta and gamma). A molecular cloning strategy involving primer-directed reverse transcription polymerase chain reaction (RT-PCR) was developed to sequence 2491 base pairs (bp) of a chicken liver–derived cDNA corresponding to the complete coding region and portions of the 5’- and 3’-untranslated regions of the chicken AMPK beta-1 mRNA. Chicken AMPK beta-1 mRNA codes for a predicted 273 amino acid protein that shares 90% homology with the human beta-1 protein. This sequence data confirmed for the first time the existence and expression of the AMPK beta-1 mRNA in chickens. Such data will be useful to scientists interested in studying the structure and expression of the chicken AMPK beta-1 gene, as well as, in further defining the genetic basis and function of the AMPK pathway in poultry.