Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 9/21/2005
Publication Date: 10/11/2005
Citation: Proszkowiec-Weglarz, M., Richards, M.P., Poch, S.M. 2005. Gallus gallus 5'-Amp-activated protein kinase, gamma-2 non-catalytic subunit, transcript variant 4 (PRKAG2) mRNA, complete cds, alternatively spliced [abstract]. GenBank Accession No. DQ212711.
Technical Abstract: AMP-activated protein kinase (AMPK) serves a ‘fuel gauge’ that responds to fluctuations in cellular energy level. AMPK exists as an enzyme complex consisting of one catalytic subunit (alpha) and two regulatory subunits (beta and gamma). A molecular cloning strategy involving primer-directed reverse transcription polymerase chain reaction (RT-PCR) was developed to sequence 1731 base pairs (bp) of a chicken breast muscle–derived cDNA corresponding to the complete coding region and portions of the 5’- and 3’-untranslated regions of the chicken AMPK gamma-2 mRNA, transcript variant 4. The absence of one exon by alternative splicing of transcript variant 3 resulted in the use of an alternative translation initiation codon and a shift in the open reading frame as compared to transcript variant 3. Chicken AMPK gamma-2, transcript variant 4 mRNA codes for a predicted 158 amino acid protein that contains only two cystathionine beta-synthase (CBS) domains (one Bateman domain). This sequence data confirmed for the first time the existence and expression of the AMPK gamma-2, variant 4 mRNA in chickens. Such data will be useful to scientists interested in studying the structure and expression of the chicken AMPK gamma-2 gene, as well as, in further defining the genetic basis and function of the AMPK pathway in poultry.