Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 9/21/2005
Publication Date: 10/11/2005
Citation: Proszkowiec-Weglarz, M., Richards, M.P., Poch, S.M. 2005. Gallus gallus 5'-AMP-activated protein kinase, gamma-2 non-catalytic subunit, transcript variant 3 (PRKAG2) mRNA, complete cds, alternatively splicedd [abstract]. GenBank Accession No. DQ212710.
Technical Abstract: AMP-activated protein kinase (AMPK) is a sensor of cellular energy charge. Once activated by an increase in the AMP/ATP ratio, AMPK acts as a master switch to increase energy-generating and to reduce energy-consuming metabolic pathways. AMPK exists as an enzyme complex consisting of one catalytic subunit (alpha) and two regulatory subunits (beta and gamma). A molecular cloning strategy involving primer-directed reverse transcription polymerase chain reaction (RT-PCR) was developed to sequence 1790 base pairs (bp) of a chicken breast muscle–derived cDNA corresponding to the complete coding region and portions of the 5’- and 3’-untranslated regions of the chicken AMPK gamma-2 mRNA, transcript variant 3. As compared to transcript variant 1, alternative splicing of the gamma-2 gene transcript resulted in the exclusion of the first four exons and in a different 5’-end of the transcript variant 3. Chicken AMPK gamma-2, transcript variant 3 mRNA codes for a predicted 328 amino acid protein that contains three cystathionine beta-synthase (CBS) domains (one complete Bateman domain). This sequence data confirmed for the first time the existence and expression of the AMPK gamma-2, transcript variant 3 mRNA in chickens. Such data will be useful to scientists interested in studying the structure and expression of the chicken AMPK gamma-2 gene, as well as, in further defining the genetic basis and function of the AMPK pathway in poultry.