Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 9/21/2005
Publication Date: 10/11/2005
Citation: Proszkowiec-Weglarz, M., Richards, M.P., Poch, S.M. 2005. Gallus gallus 5'-AMP-activated protein kinase gamma-2 non-catalytic subunit transcript variant 1, (PRKAG2) mRNA, complete cds, alternatively spliced [abstract]. GenBank Accession No. DQ212708.
Technical Abstract: AMP-activated protein kinase (AMPK) is a sensor of cellular energy charge. Once activated by an increase in the AMP:ATP ratio, AMPK acts as a master switch to increase energy-generating and to reduce energy-consuming metabolic pathways. AMPK exists as an enzyme complex consisting of one catalytic subunit (alpha) and two regulatory subunits (beta and gamma). A molecular cloning strategy involving primer-directed reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of 5’ cDNA ends (5’ RACE) was developed to sequence 2488 base pairs (bp) of a chicken liver–derived cDNA corresponding to the complete coding region and portions of the 5’- and 3’-untranslated regions of the chicken AMPK gamma-2 mRNA transcript variant 1. The chicken AMPK gamma-2 transcript variant 1 mRNA codes for a predicted 567 amino acid protein which contains four cystathionine beta-synthase (CBS) domains (two Bateman domains). This sequence data confirmed for the first time the existence and expression of the AMPK gamma-2 transcript variant 1 mRNA in chickens. Such data will be useful in studying the structure and expression of the chicken AMPK gamma-2 gene, as well as, in further defining the function of the AMPK pathway in poultry.