Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 7/19/2005
Publication Date: 8/1/2005
Citation: Proszkowiec-Weglarz, M., Richards, M.P., Poch, S.M. 2005. Gallus gallus 5'-AMP-activated protein kinase gamma-1 non-catalytic subunit (PRKAG1), transcript variant 2, mRNA, complete cds. GenBank Accession Number DQ133598.
Technical Abstract: AMP-activated protein kinase (AMPK) serves a ‘fuel gauge’ that responds to fluctuations in cellular energy level. AMPK exists as an enzyme complex consisting of one catalytic subunit (alpha) and two regulatory subunits (beta and gamma). A molecular cloning strategy involving primer-directed reverse transcription polymerase chain reaction (RT-PCR) was developed to sequence 887 base pairs (bp) of a chicken liver–derived cDNA corresponding to the complete coding region and portions of the 5’- and 3’-untranslated regions of the chicken AMPK gamma-1 mRNA, transcript variant 2. The absence of one exon by alternative splicing of transcript variant 2 resulted in the use of an alternative translation initiation codon and a shift in the open reading reading frame as compared to transcript variant 1. Chicken AMPK gamma-1, transcript variant 2 mRNA codes for a predicted 276 amino acid protein that contains three cystathionine beta-synthase (CBS) domains (1 complete Bateman domain). This sequence data confirmed for the first time the existence and expression of the AMPK gamma-1, variant 2 mRNA in chickens. Such data will be useful to scientists interested in studying the structure and expression of the chicken AMPK gamma-1 gene, as well as, in further defining the genetic basis and function of the AMPK pathway in poultry.