Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 7/19/2005
Publication Date: 8/1/2005
Citation: Proszkowiec-Weglarz, M., Richards, M.P. Poch, S.M. 2005. Gallus gallus 5'AMP-activated protein kinase gamma-1 non-catalytic subunit (PRKAG1), transcript variant 1, mRNA, complete cds. GenBank Accession Number DQ133597.
Technical Abstract: AMP-activated protein kinase (AMPK) is a sensor of cellular energy charge. Once activated by an increase in the AMP/ATP ratio, AMPK acts as a master switch to increase energy-generating and to reduce energy-consuming metabolic pathways. AMPK exists as an enzyme complex consisting of one catalytic subunit (alpha) and two regulatory subunits (beta and gamma). A molecular cloning strategy involving primer-directed reverse transcription polymerase chain reaction (RT-PCR) was developed to sequence 991 base pairs (bp) of a chicken liver–derived cDNA corresponding to the complete coding region and portions of the 5’- and 3’-untranslated regions of the chicken AMPK gamma-1 mRNA, transcript variant 1. As compared to transcript variant 2, the presence of an additional exon from the AMPK gamma-1 gene resulted in a shift of the open reading frame of transcript variant 1 through the use of a different translation initiation codon. Chicken AMPK gamma-1, variant 1 mRNA codes for a predicted 298 amino acid protein that contains four cystathionine beta-synthase (CBS) domains (2 Bateman domains). This sequence data confirmed for the first time the existence and expression of the AMPK gamma-1, transcript variant 1 mRNA in chickens. Such data will be useful to scientists interested in studying the structure and expression of the chicken AMPK gamma-1 gene, as well as, in further defining the genetic basis and function of the AMPK pathway in poultry.